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Updated: Jun 11, 2025
09:23
Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
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CLAVATA3 INSENSITIVE RECEPTOR KINASEs regulate lateral root initiation and spacing in Arabidopsis
Xianghu Meng1, Rui Ye1, Jing Cao1
1Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Plant Physiology
|October 10, 2024
Summary
CLAVATA3 INSENSITIVE RECEPTOR KINASEs (CIKs) and RECEPTOR-LIKE KINASE 7 (RLK7) perceive the TARGET OF LBD SIXTEEN 2 (TOLS2) signal. This interaction regulates lateral root initiation and spacing via the MKK4/5-MPK3/6-PUCHI pathway.
Area of Science:
- Plant Biology
- Molecular Plant Science
- Developmental Biology
Background:
- Lateral root (LR) development is crucial for plant adaptation to environmental changes.
- RECEPTOR-LIKE KINASE 7 (RLK7) and its ligand TARGET OF LBD SIXTEEN 2 (TOLS2) are known regulators of LR initiation and spacing.
- The precise molecular mechanisms of TOLS2 signal perception and transduction by RLK7 are not fully understood.
Purpose of the Study:
- To investigate the role of CLAVATA3 INSENSITIVE RECEPTOR KINASEs (CIKs) in Arabidopsis lateral root development.
- To elucidate the interaction between CIKs and the RLK7-mediated signaling pathway in regulating LR initiation and spacing.
Main Methods:
- Phenotypic analysis of various cik mutants in Arabidopsis thaliana.
- Biochemical assays to examine interactions between CIKs, RLK7, and other signaling components.
- Analysis of TOLS2 peptide sensitivity in cik mutants.
- Investigating the role of auxin, PUCHI, MKK4, and MKK5 in LR development.
Main Results:
- High-order cik mutants exhibited increased LR initiation and defective LR spacing, with reduced sensitivity to TOLS2.
- TOLS2 application enhanced CIK-RLK7 interactions and RLK7-dependent CIK phosphorylation.
- Overexpression of PUCHI and activation of MKK4/MKK5 partially rescued LR spacing defects in cik and rlk7-3 mutants.
- Auxin maxima influenced CIK subcellular localization, determining lateral root founder cells.
Conclusions:
- CIKs and RLK7 act in concert to perceive the TOLS2 signal, regulating LR initiation and spacing.
- The signaling pathway involves MKK4/5, MPK3/6, and the transcription factor PUCHI.
- CIK subcellular localization, modulated by auxin, plays a role in specifying lateral root founder cells.
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