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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Jun 11, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Barcode 100K Specimens: In a Single Nanopore Run.

Paul D N Hebert¹, Robin Floyd¹, Saeideh Jafarpour¹

¹Centre for Biodiversity Genomics, University of Guelph, Guelph, Ontario, Canada.

Molecular Ecology Resources

|October 10, 2024

Summary

This summary is machine-generated.

DNA barcoding advances species identification for biosphere management. New nanopore sequencing technology drastically cuts costs, making large-scale animal species census feasible at $0.01 per specimen.

Keywords:

Bioinfomatics/Phyloinfomatics DNA barcoding amplicon sequencing insects molecular evolution

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Last Updated: Jun 11, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

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Published on: May 23, 2018

Area of Science:

Biodiversity research
Genomic technologies
Conservation science

Background:

Global biosphere management requires comprehensive species data, but high costs limit data acquisition.
DNA barcoding automates animal species recognition via the BIN system, but requires affordable sequencing.
Previous high-throughput sequencing platforms were expensive, limiting accessibility.

Purpose of the Study:

To evaluate the cost-effectiveness and feasibility of using Oxford Nanopore Technologies (ONT) sequencers for large-scale DNA barcoding.
To demonstrate that recent advancements in ONT flow cell technology enable high-throughput, low-cost DNA sequencing for biodiversity studies.

Main Methods:

Utilized ONT MinION and Flongle flow cells for DNA sequencing of pooled amplicons.
Characterized amplicon pools representing up to 100,000 specimens per MinION flow cell and several thousand per Flongle flow cell.
Calculated the cost per specimen for the DNA sequencing step.

Main Results:

ONT's latest R10.4.1 flow cells offer improved fidelity, overcoming previous adoption barriers.
A single MinION flow cell successfully characterized pooled samples from 100,000 specimens.
A Flongle flow cell processed pooled samples from several thousand specimens.
The cost of DNA sequencing was reduced to $0.01 per specimen, becoming the least expensive step in the workflow.

Conclusions:

Oxford Nanopore sequencing technology, particularly with R10.4.1 flow cells, provides a cost-effective solution for large-scale DNA barcoding.
This technological advancement significantly lowers the barrier to conducting comprehensive species censuses, aiding biosphere management.
DNA sequencing is now economically viable for analyzing billions of specimens required for a complete animal species inventory.