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Published on: October 23, 2009
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Photonic Microbead Array Digital Time-Resolved Fluorescence Ultrasensitive Platform for Simultaneous Detection of
Yuting Zhu1, Wei Li2, Jing Yang1
1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China.
Analytical Chemistry
|October 10, 2024
Summary
A new time-resolved fluorescence immunoassay (TRFIA) platform using europium nanoparticles on photonic microbead arrays offers highly sensitive, multi-target mycotoxin detection. This advancement significantly improves upon traditional methods for rapid food safety screening.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Food Science
Background:
- Lateral flow immunoassays face limitations in sensitivity, linear range, and cross-reactivity for multi-target screening.
- Mycotoxin contamination in food poses significant health risks, necessitating sensitive and rapid detection methods.
Purpose of the Study:
- To develop a novel time-resolved fluorescence immunoassay (TRFIA) platform for sensitive, multiplexed detection of mycotoxins.
- To overcome the limitations of existing immunoassay technologies for rapid food safety screening.
Main Methods:
- Conjugation of europium chelate polystyrene (PS@Eu) nanoparticles with monoclonal antibodies for mycotoxin sensing.
- Utilizing a competitive TRFIA protocol on photonic microbead arrays (PMAs) with a digital time-resolved fluorescence reader.
- Evaluating performance metrics including linear detection ranges, limits of detection (LODs), specificity, recovery, and reproducibility in grain samples.
Main Results:
- The developed TRFIA demonstrated wide linear detection ranges (0.01-1000 ng/mL for DON, 0.1-100 ng/mL for OTA, 0.01-100 ng/mL for AFB1) and low LODs (7.9 pg/mL for DON, 18 pg/mL for OTA, 7.7 pg/mL for AFB1).
- Photonic microbead arrays (PMAs) enhanced the fluorescence signal approximately twofold due to increased electric field distribution.
- The new TRFIA was 1000-fold more sensitive than classical TRFIA, showing good specificity, recovery (76.68-117.26%), and reproducibility in grain samples.
Conclusions:
- The novel TRFIA platform effectively overcomes limitations of traditional immunoassays for multi-mycotoxin detection.
- This platform exhibits high sensitivity, broad linear ranges, and excellent performance in real samples, indicating significant potential for rapid food safety screening.
- The enhanced fluorescence from PMAs contributes to the superior sensitivity of this advanced immunoassay system.
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