A purine fluorescent derived probe assay for glyphosate and mesotrione via Schiff base cleavage

A fluorescent probe derived from purine with Schiff base moiety was developed for the recognization of glyphosate and mesotrione. The detected glyphosate and mesotrione can lead to the dissociation of the Schiff base probe to enhance the fluorescence via a turn-off PET process. Mechanism study revealed that the synergistic effect of the phosphoric acid and the secondary amine moieties in glyphosate results in the bond cleavage of the Schiff base probe. Quantitative measurements of glyphosate and mesotrione were achieved with the detection limits of 17.2 nM and 484.32 nM, respectively. Meanwhile, the detection of glyphosate pesticide in real samples and cells was also conducted, demonstrating the good practicality and cytocompatibility of the probe.