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Updated: Jun 10, 2025

Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens
Published on: March 14, 2019
The new chassis in the flask: Advances in Vibrio natriegens biotechnology research
Matthew Lima1, Charandatta Muddana2, Zhengyang Xiao1
1Department of Energy, Environmental, and Chemical Engineering, Washington University in St. Louis, 1 Brookings Drive, St. Louis, MO 63130, USA.
Abstract:
Biotechnology has been built on the foundation of a small handful of well characterized and well-engineered organisms. Recent years have seen a breakout performer gain attention as a new entrant into the bioengineering toolbox: Vibrio natriegens. This review covers recent research efforts into making V. natriegens a biotechnology platform, using a large language model (LLM) and knowledge graph to expedite the literature survey process. Scientists have made advancements in research pertaining to the fundamental metabolic characteristics of V. natriegens, development and characterization of synthetic biology tools, systems biology analysis and metabolic modeling, bioproduction and metabolic engineering, and microbial ecology. Each of these subcategories has relevance to the future of V. natriegens for bioengineering applications. In this review, we cover these recent advancements and offer context for the impact they may have on the field, highlighting benefits and drawbacks of using this organism. From examining the recent bioengineering research, it appears that V. natriegens is on the precipice of becoming a platform bacterium for the future of biotechnology.
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