Fabrication and Fluorescence Analysis of Rhodamine Dyes in Polycarbonate Serpentine Microfluidic System

The synthesis of rhodamine dyes (R6G and R1010) and their fluorescence characterization within polymer-based microfluidics, offers an exciting and novel approach in materials science and chemical analysis. This work investigates the emission of polycarbonate substrates (PC) by UV-visible. The ablation threshold (16mj.sec^-1) of PC at 193nm wavelength after that ablation process continued to produce microfluidic serpentine channels on PC by using G-Code. The fluorescence characteristics of Rhodamine 6G and Rhodamine 101 are investigated. Absorption and emission at peak wavelength were analyzed against R6G and R101 concentrations. Furthermore, the refractive indices of both R6G and R101 vis concentrations are examined. As a result at low concentrations, there was the highest overlapping, and at high concentrations, there was the smallest overlapping. R101 showed better photostability and a more consistent diffusion, whereas R6G had a faster diffusion and stronger fluorescence intensity. These differences were caused by the different molecular structures of the dyes and their interactions with the PC microchannel. Incorporating R6G and R101 dyes into a polycarbonate PC microfluidic chip would enhances both the resolution and sensitivity of fluorescence detection. The limited microfluidic setup facilitates ultra-high-resolution investigation and minimizing sample volumes, making it suitable for applications requiring precise measurements. The innovation relies on the utilization of the unique fluorescence characteristics of R6G (Rhodamine 6G) and R101 (Rhodamine 101) dyes to enhance the performance of polycarbonate microfluidic devices. R6G has high fluorescence quantum yield and stability, rendering it suitable for sensitive detection, while R101 offers superior brightness and improved resistance to photobleaching. Incorporation of these dyes into polymeric microfluidics improves sensitivity and facilitates real-time, dynamic sample analysis. This method offers a portable, economical solution with high-throughput capabilities, greatly enhancing both analytical and process accuracy across a variety of applications.