Aged rather than pristine polyvinyl chloride microplastic affect the development and structure of Vallisneria natans...

A large number of microplastics have been discharged into freshwater ecosystems, where they age and are deposited in the sediment, posing a risk to primary producers, such as submerged macrophytes. Many macrophytes benefit from clonal integration, which lets the population work as a 'macro' organism. Nonetheless, little is known about the differences in phytotoxicity between aged and pristine microplastics, particularly for clonal populations of macrophytes. In this study, we showed that UV-aging changes the characteristics of polyvinyl chloride microplastics (PVC-MPs). Aged PVC-MPs possessed higher hydrophilicity, less chlorine and crystallinity, and more severe toxicity. The pristine PVC-MPs did not affect Vallisneria natans, while the aged PVC-MPs significantly affected the development and structure of the clonal population. The severely aged PVC-MPs reduced the relative growth rate of V. natans by 26 % at the population level. Furthermore, the mother plant (ortet) and offspring (ramet) responded differently to the aged PVC-MPs. A trade-off was observed between the growth rate and stress resistance in the ortets. The ortets increased investment in the root part to tolerate stress when facing exposure to microplastics. In contrast, the ramets were less resistant, as shown by shorter roots, and lower leaf chlorophyll, carbon, and nitrogen concentrations. Notably, the growth of the ramets was maintained and the investments in stolon structure by the ortets were not lessened. The ortet sacrificed itself for the continuation of the ramet. This clonal integration may safeguard V. natans survival and compensate for vegetative expansion. This study sheds new light on how macrophytes respond to microplastics at the clonal population level and provides direct evidence that existing studies may have underestimated the toxic effect of microplastics in freshwater ecosystems.