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Related Experiment Video

Updated: Jun 10, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Published on: October 6, 2023

Single-shot 20-fold expansion microscopy.

Shiwei Wang^1,2, Tay Won Shin^1,3, Harley B Yoder^1,4,5

¹McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, USA.

|October 11, 2024

Summary

This summary is machine-generated.

We developed 20ExM, a novel expansion microscopy (ExM) technique. This single-step protocol achieves ~20× physical magnification for nanoscale imaging on conventional microscopes.

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Area of Science:

Biotechnology
Microscopy
Cell Biology

Background:

Expansion microscopy (ExM) enables nanoscale imaging using conventional microscopes.
Current ExM methods offer limited expansion (~4-10×) or require iterative steps for higher magnification (~15-20×).

Purpose of the Study:

To introduce a simplified ExM protocol achieving high linear expansion in a single step.
To enable nanoscale resolution (<20 nm) with standard microscopy equipment.

Main Methods:

Developed a novel single-step expansion microscopy protocol, termed 20ExM.
Achieved approximately 20× linear physical magnification of biological specimens.

Main Results:

The 20ExM protocol provides <20-nm resolution on conventional microscopes.
This method simplifies high-magnification ExM, matching iterative approaches in performance.
20ExM supports postexpansion staining, particularly for brain tissue.

Conclusions:

20ExM offers a single-step solution for high-resolution nanoscale imaging.
The protocol facilitates biomolecular labeling and has broad applications in biological research.
This technique enhances the utility of conventional microscopes for advanced imaging.