Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chemical Probes to Reveal the Assembly and Dynamics of Wall Teichoic Acids.

Journal of the American Chemical Society·2026

Same author

Isotropic shrinkage of patterned vacancies enables three-dimensional nanoprecise metastructures for visible light applications.

Nature photonics·2026

Same author

Intelectin-2 is a broad-spectrum antimicrobial lectin.

Nature communications·2026

Same author

Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs.

bioRxiv : the preprint server for biology·2025

Same author

Deep learning the dynamic regulatory sequence code of cardiac organoid differentiation.

bioRxiv : the preprint server for biology·2025

Same author

Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context.

bioRxiv : the preprint server for biology·2025

Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026

Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026

Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026

Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026

Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026

Same journal

3D pathology-guided microdissection.

Nature methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 10, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Published on: October 6, 2023

Single-shot 20-fold expansion microscopy.

Shiwei Wang^1,2, Tay Won Shin^1,3, Harley B Yoder^1,4,5

¹McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, USA.

|October 11, 2024

Summary

This summary is machine-generated.

We developed 20ExM, a novel expansion microscopy (ExM) technique. This single-step protocol achieves ~20× physical magnification for nanoscale imaging on conventional microscopes.

More Related Videos

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Published on: April 28, 2023

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

Related Experiment Videos

Last Updated: Jun 10, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Published on: October 6, 2023

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Published on: April 28, 2023

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

Area of Science:

Biotechnology
Microscopy
Cell Biology

Background:

Expansion microscopy (ExM) enables nanoscale imaging using conventional microscopes.
Current ExM methods offer limited expansion (~4-10×) or require iterative steps for higher magnification (~15-20×).

Purpose of the Study:

To introduce a simplified ExM protocol achieving high linear expansion in a single step.
To enable nanoscale resolution (<20 nm) with standard microscopy equipment.

Main Methods:

Developed a novel single-step expansion microscopy protocol, termed 20ExM.
Achieved approximately 20× linear physical magnification of biological specimens.

Main Results:

The 20ExM protocol provides <20-nm resolution on conventional microscopes.
This method simplifies high-magnification ExM, matching iterative approaches in performance.
20ExM supports postexpansion staining, particularly for brain tissue.

Conclusions:

20ExM offers a single-step solution for high-resolution nanoscale imaging.
The protocol facilitates biomolecular labeling and has broad applications in biological research.
This technique enhances the utility of conventional microscopes for advanced imaging.