Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

743
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
743
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

657
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
657
Mass Spectrometers01:16

Mass Spectrometers

5.2K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.2K
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

282
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
282
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

732
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
732
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

611
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
611

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From performance to practice: knowledge-distilled segmentator for on-premises clinical workflows.

npj health systems·2026
Same author

Cognitive engagement induces area-specific fingerprints of dopamine, acetylcholine, serotonin, glutamate and GABA in prefrontal cortex and striatum.

bioRxiv : the preprint server for biology·2026
Same author

Nonexhaustive microextraction as a step toward more sustainable chemical analysis in the field and the clinic.

Nature protocols·2026
Same author

<i>In Vivo</i> Negligible Depletion SPME for the Determination of Free and Total Concentrations of Anandamide and 2-Arachidonoylglycerol in the Brain of a Parkinson's Disease Rat Model.

Analytical chemistry·2026
Same author

ETT-CKGE: Efficient Task-driven Tokens for Continual Knowledge Graph Embedding.

Machine learning and knowledge discovery in databases : European Conference, ECML PKDD ... : proceedings. ECML PKDD (Conference)·2026
Same author

Defects in auxiliary fuel oxidation and mitochondrial pyruvate transport mark transition to overt heart failure in Tgαq*44 mice.

Journal of translational medicine·2026
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026
Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026
Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026
Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 10, 2025

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
09:22

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

14.2K

3D Printed Coated Blade Spray-Mass Spectrometry Devices.

Wei Zhou1, Qizhen Lan1, Malvika Dutt1

  • 1Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

Analytical Chemistry
|October 12, 2024
PubMed
Summary
This summary is machine-generated.

3D printing enables user-friendly solutions for coated blade spray-mass spectrometry (CBS-MS). New interfaces and extraction devices accelerate trace compound screening in biological samples like blood and urine.

More Related Videos

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.1K
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

9.4K

Related Experiment Videos

Last Updated: Jun 10, 2025

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
09:22

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

14.2K
Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.1K
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

9.4K

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • 3D Printing Applications

Background:

  • Coated blade spray-mass spectrometry (CBS-MS) offers rapid trace compound screening in complex biological samples.
  • Widespread adoption of CBS-MS is limited by the unavailability of user-friendly mass spectrometry (MS) interfaces and extraction devices.

Purpose of the Study:

  • To develop and validate comprehensive 3D-printed solutions for CBS-MS, including a novel MS interface and two distinct extraction devices.
  • To address the need for accessible and efficient sample preparation in trace analysis using CBS-MS.

Main Methods:

  • Fabrication of a versatile MS interface with a separated immobilization station and blade holder using 3D printing.
  • Development of two 3D-printed extraction devices: one for large-volume LC vial samples (48 samples/20s) and another for single-drop blood analysis (8 samples).
  • Evaluation of the developed devices for rapid screening of seven drugs in urine and single-drop blood samples, including assessment of potential contamination from 3D-printed materials.

Main Results:

  • The novel MS interface simplifies workflow and reduces contamination risks.
  • High-throughput sample preparation achieved with extraction cartridges (average <20s per sample).
  • Successful rapid screening of seven drugs in urine and blood samples demonstrated promising analytical performance.

Conclusions:

  • 3D printing provides effective solutions for creating user-friendly and efficient CBS-MS interfaces and extraction devices.
  • The developed 3D-printed tools enhance the accessibility and applicability of CBS-MS for trace compound analysis.
  • Careful material selection and testing are crucial to prevent contamination issues with 3D-printed components in MS analysis.