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Updated: May 7, 2026
Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
Published on: April 18, 2013
Hydrogen-Bonded Organic Framework Enhanced Antifouling Property for Efficient In Situ Electrochemical Assay of
Zhiyuan Zheng1, Ningxuan Liu1, Jiajia Lu1
1NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
Abstract:
Accurate determination of cerebral ascorbic acid (AA) is crucial for understanding ischemic stroke (IS) related pathological events. Carbon fiber microelectrodes (CFEs) have proven to be robust tools with high sensitivity toward AA, however, they face ongoing challenges for in situ measurement due to the non-specific adsorption of proteins in brain tissue. In this study, the hydrogen-bonded organic framework PFC-71 is synthesized and modified on CFEs through π-π stacking interactions with carboxylated carbon nanotubes (CNT-COOH). It is found that the gating effect and hydrophilicity of PFC-71 provided the CFE with excellent antibiofouling properties. As a result, AA exhibited a low oxidation potential of -30 mV on the CFE/CNT-COOH/PFC-71, even in the presence of 20 mg mL-1 bovine serum albumin. Given the structural advantages of CFE/CNT-COOH/PFC-71, a ratiometric electrochemical strategy for AA is established, enabling the in situ assay of cerebral AA in a middle cerebral artery occlusion (MCAO) model with high accuracy and stability.
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