Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond the Bedside: Acute Care Providers' Awareness of Food Insecurity and Available Resources.

Journal of nutrition education and behavior·2026
Same author

Characterizing the MSMP-CCR2 Interaction through Molecular Dynamics Simulations and Machine Learning Approaches.

Journal of chemical information and modeling·2026
Same author

Exploring the association between common genetic deletions and aging: insights from the Canadian Longitudinal Study on Aging.

GeroScience·2026
Same author

META-DIFF: a k-mer-based pipeline that detects differentially abundant sequences in metagenomics whole genome sequencing.

Scientific reports·2026
Same author

Editorial: Advances in systems biology and bioinformatics for microbiology research.

Canadian journal of microbiology·2026
Same author

Cell-Free DNA Uncovered Tumor Heterogeneity and Informed Targeted Therapy in a Pediatric Rhabdomyosarcoma.

JCO precision oncology·2026

Related Experiment Video

Updated: Jun 10, 2025

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

3.6K

Ruminant Trans Fatty Acid Intake Modulates Inflammation Pathways in the Adipose Tissue Transcriptome of C57BL/6 Mice.

Farzad Mohammadi1,2, Charles Joly Beauparlant1, Stéphanie Bianco1

  • 1Endocrinology and Nephrology Unit, CHU de Québec-Université Laval Research Center, Québec, Canada.

Molecular Nutrition & Food Research
|October 13, 2024
PubMed
Summary

Trans-palmitoleic acid (TPA) significantly alters gene expression in adipose tissue compared to elaidic acid (EA), impacting inflammation and glucose metabolism pathways. This highlights TPA

Keywords:
adipose tissuebioinformaticsglucose metabolisminflammationtrans fatstranscriptomicstype 2 diabetes

More Related Videos

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.0K
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

2.7K

Related Experiment Videos

Last Updated: Jun 10, 2025

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

3.6K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.0K
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

2.7K

Area of Science:

  • Nutritional Science
  • Molecular Biology
  • Genomics

Background:

  • Dietary trans fatty acids (TFAs) have diverse physiological effects.
  • Elaidic acid (EA) and trans-palmitoleic acid (TPA) are common TFAs with distinct metabolic profiles.
  • Understanding their differential impact on adipose tissue is crucial for metabolic health.

Purpose of the Study:

  • To compare the transcriptomic effects of elaidic acid (EA) and trans-palmitoleic acid (TPA) in adipose tissue.
  • To elucidate the distinct roles of EA and TPA in regulating inflammation and glucose metabolism.
  • To identify specific genes and pathways affected by TPA consumption.

Main Methods:

  • C57BL/6 mice were administered EA or TPA via nanovesicles in drinking water for 28 days.
  • Mice were fed a regular fat diet (18% calories from fat).
  • Total RNA was extracted from adipose tissue, followed by paired-end sequencing to analyze transcriptomic profiles.

Main Results:

  • Trans-palmitoleic acid (TPA) intake altered the expression of 351 genes compared to elaidic acid (EA) intake (340 upregulated, 11 downregulated; FC >1.5, p < 0.05).
  • Upregulated genes in TPA vs. EA included Slc5a8, Lcn2, Csf3, and Mapk13.
  • Affected pathways included NOD-like receptor signaling, lipid metabolism, IL-17 signaling, TNF signaling, and insulin resistance.

Conclusions:

  • Trans-palmitoleic acid (TPA) has a distinct regulatory impact on inflammation and diabetes-related pathways in adipose tissue compared to elaidic acid (EA).
  • These findings provide molecular insights into the differential metabolic effects of various trans fatty acids.