PFDN5 plays a dual role in breast cancer and regulates tumor immune microenvironment: Insights from integrated bioinformatics analysis and experimental validation
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View abstract on PubMed
Summary
This summary is machine-generated.Prefoldin 5 (PFDN5) has a dual role in breast cancer, affecting cell cycle and migration. Lower PFDN5 expression correlates with poorer prognosis and impacts the tumor immune microenvironment, making it a potential biomarker.
Area Of Science
- Oncology
- Molecular Biology
- Immunology
Background
- Breast cancer remains a leading cause of death globally, despite improved prognoses.
- Prefoldin 5 (PFDN5), a prefoldin complex subunit, is crucial for protein folding.
- The precise role of PFDN5 in breast cancer development and prognosis is not well understood.
Purpose Of The Study
- To investigate the correlation between PFDN5 expression and breast cancer patient survival and clinicopathological features.
- To elucidate the biological functions and molecular mechanisms of PFDN5 in breast cancer.
- To assess the impact of PFDN5 on the tumor immune microenvironment.
Main Methods
- Bioinformatics analysis of PFDN5 expression and survival data.
- In vitro assays to determine PFDN5's functional roles in breast cancer cells.
- RNA sequencing (RNA-seq) to identify associated molecular pathways.
- Analysis of PFDN5's effect on tumor immune cell infiltration and polarization.
Main Results
- PFDN5 expression is lower in breast cancer tissues and associated with poorer prognosis.
- PFDN5 induces G2/M cell cycle arrest and reduces proliferation but promotes migration and invasion.
- RNA-seq identified PFDN5 involvement in cell cycle and TGF-β signaling.
- PFDN5 influences the tumor immune microenvironment by promoting M1 macrophage polarization and increasing CD8+ T cell infiltration.
Conclusions
- PFDN5 exhibits a dual role in breast cancer, influencing both tumor progression and the immune microenvironment.
- PFDN5 is a key factor in modulating the tumor immune microenvironment.
- PFDN5 shows potential as a predictive biomarker for metastasis and prognosis in breast cancer.
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