In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles

Area of Science:

• Reproductive biology
• Nanotechnology applications
• Assisted Reproductive Technologies (ARTs)

Background:

• Nanoparticle (NP) applications are expanding into reproductive science.
• Oviductin (OVGP1) functionalized NPs offer novel labeling for oocytes in ARTs.
• Investigating NP toxicity in gametes is crucial for ART safety.

Purpose of the Study:

• To evaluate the impact of OVGP1-functionalized NPs (NPOv) on boar sperm functionality.
• To assess sperm quality parameters after exposure to NPOv in different media.
• To determine if NPOv on oocytes affects sperm-zona pellucida (ZP) binding.

Main Methods:

• Boar sperm were co-incubated with NPOv in BTS and TALP media for varying durations.
• Sperm viability, motility, and kinematics were analyzed.
• Sperm attachment to NPOv-labeled oocyte ZP was assessed.

Main Results:

• No significant differences in sperm viability, motility, or kinematics were observed in TALP medium.
• While some kinetic parameters changed in BTS, overall motility and viability remained unaffected.
• NPs on the oocyte ZP did not impede sperm attachment.

Conclusions:

• In vitro exposure of boar sperm to OVGP1-functionalized NPs does not impair sperm functionality.
• NPs are safe for use in IVF media and on oocyte surfaces for ART applications.
• This study supports the use of NPOv for oocyte labeling in porcine ARTs.