Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Energy to Drive Translocation01:37

Energy to Drive Translocation

2.0K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.0K
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

3.3K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.3K
Mitochondrial Membranes01:45

Mitochondrial Membranes

9.1K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
9.1K
Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

3.3K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
3.3K
The Movement of Organelles and Vesicles01:43

The Movement of Organelles and Vesicles

4.4K
In eukaryotic cells,  cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
4.4K
Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

4.3K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RIVA: An Image Dataset of Conventional Pap Smear Cytology with Multiple Independent Annotations.

Scientific data·2025
Same author

Dynamical organization of vimentin intermediate filaments in living cells revealed by MoNaLISA nanoscopy.

Bioscience reports·2025
Same author

Inference of the force pattern acting on a semiflexible filament from shape analysis.

Physical review. E·2024
Same author

From the membrane to the nucleus: mechanical signals and transcription regulation.

Biophysical reviews·2023
Same author

Author Correction: Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks.

Scientific reports·2023
Same author

Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks.

Scientific reports·2023

Related Experiment Video

Updated: Jun 10, 2025

Studying Mitochondrial Structure and Function in Drosophila Ovaries
09:53

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Published on: January 4, 2017

23.9K

Deciphering the intracellular forces shaping mitochondrial motion.

Agustina Belén Fernández Casafuz1, Azul Marí A Brigante2, Marí A Cecilia De Rossi3,4

  • 1CONICET - Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Instituto de Cálculo (IC), Buenos Aires, 1428, Argentina. afcasafuz@ic.fcen.uba.ar.

Scientific Reports
|October 13, 2024
PubMed
Summary

We developed a new, nearly non-invasive method to quantify mechanical forces on mitochondria in living cells. This tool detects localized impulses, revealing how cytoskeletal interactions influence mitochondrial dynamics and function.

More Related Videos

Author Spotlight: Decoding Mitochondrial Aging
08:48

Author Spotlight: Decoding Mitochondrial Aging

Published on: June 30, 2023

3.6K
Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
07:55

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

1.3K

Related Experiment Videos

Last Updated: Jun 10, 2025

Studying Mitochondrial Structure and Function in Drosophila Ovaries
09:53

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Published on: January 4, 2017

23.9K
Author Spotlight: Decoding Mitochondrial Aging
08:48

Author Spotlight: Decoding Mitochondrial Aging

Published on: June 30, 2023

3.6K
Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
07:55

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

1.3K

Area of Science:

  • Cell Biology
  • Biophysics
  • Mechanobiology

Background:

  • Mechanical forces significantly impact mitochondrial dynamics, morphology, and distribution within cells.
  • The precise mechanisms by which cytoskeletal interactions influence mitochondrial function remain largely unknown.

Purpose of the Study:

  • To introduce a novel quantitative method for exploring forces affecting mitochondria in living cells.
  • To detect localized mechanical impulses on mitochondria amidst thermal fluctuations.
  • To extend knowledge on quantifying mechanical cue impact at the single organelle level.

Main Methods:

  • Development of a novel, nearly non-invasive quantitative technique.
  • Detection of localized mechanical impulses on mitochondria.
  • Distinguishing mechanical forces from thermal jittering in the cytoplasm.

Main Results:

  • The study highlights the distinct roles of cytoskeletal networks in shaping mitochondrial dynamics.
  • The new method enables the detection of mechanical forces acting on individual mitochondria.
  • Demonstrated the ability to quantify mechanical influences at the single organelle level.

Conclusions:

  • The developed technique offers a new way to study the impact of mechanical forces on mitochondria.
  • This approach can be expanded to investigate other organelles and biopolymers.
  • Provides insights into the mechanisms underlying cytoskeletal-mitochondrial crosstalk.