Inflammation-Associated Long Non-Coding RNAs (lncRNAs) in Chronic Viral Hepatitis- Associated Hepatocellular Carcinoma
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View abstract on PubMed
Summary
This summary is machine-generated.Inflammation-associated long non-coding RNAs (lncRNAs) show altered expression in chronic viral hepatitis (CVH) and associated liver cancer (CVH-HCC). DLEU2, SNHG16, LINC00662, and XIST are potential diagnostic markers for CVH-HCC.
Area Of Science
- Molecular biology
- Hepatology
- Oncology
Background
- Chronic viral hepatitis (CVH) poses a significant risk for developing hepatocellular carcinoma (HCC).
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in various diseases, including cancer.
- Understanding the expression patterns of inflammation-associated lncRNAs in CVH and CVH-HCC is crucial for identifying potential biomarkers.
Purpose Of The Study
- To investigate the expression profiles of inflammation-associated lncRNAs in chronic viral hepatitis (CVH) and CVH-associated hepatocellular carcinoma (CVH-HCC).
- To evaluate the prognostic significance of these lncRNAs in the context of CVH and CVH-HCC.
Main Methods
- Real-time polymerase chain reaction (RT-PCR) was employed to analyze the expression of 84 inflammation-associated lncRNAs in formalin-fixed paraffin-embedded (FFPE) tissue samples.
- Expression levels were compared across normal liver tissue, cirrhotic CVH parenchyma, peritumoral cirrhotic parenchyma, and CVH-HCC samples.
- Specific lncRNAs (DLEU2, HOTAIR, LINC00635, LINC00662, RP11-549J18.1, SNHG16, and XIST) were further validated using RT-PCR in a larger sample set.
Main Results
- A significant upregulation of 56 inflammation-associated lncRNAs was observed in peritumoral cirrhotic parenchyma compared to normal liver tissue.
- Expression of 71 lncRNAs was notably higher in peritumoral cirrhotic parenchyma versus cirrhotic CVH parenchyma.
- DLEU2 and SNHG16 were upregulated in both tumor and peritumoral cirrhotic parenchyma relative to cirrhotic CVH parenchyma. LINC00662 was significantly higher in CVH-HCC. XIST showed increased expression in tumor and peritumoral samples without statistical significance. No significant association between lncRNA expression and survival was found.
Conclusions
- The inflammation-associated lncRNAs DLEU2, SNHG16, LINC00662, and XIST are identified as potential diagnostic biomarkers for CVH-HCC.
- Further research is required to establish their utility as prognostic markers in CVH-HCC.
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