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Related Experiment Video

Updated: Jun 10, 2025

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Bacterial Lysate-Based Bifunctional mRNA Nanoformulation for Efficient Colon Cancer Immunogene Therapy.

Xiaohua Chen¹, Jieping Wu¹, Bailing Zhou¹

¹State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.

ACS Applied Materials & Interfaces

|October 14, 2024

Summary

This study introduces a novel bifunctional mRNA delivery system using Lactobacillus reuteri lysate to enhance cancer immunotherapy. The system demonstrated high efficiency in delivering IL-23A mRNA and significantly inhibited tumor growth in preclinical models.

Keywords:

IL-23A Lactobacillus reuteri lysate immunogene therapy intestinal flora mRNA delivery

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Published on: January 20, 2023

Area of Science:

Biomedical Engineering
Immunology
Nanotechnology

Background:

mRNA-based nonviral gene therapy shows promise for cancer treatment but faces challenges in delivery efficiency and therapeutic capacity.
Immunogene therapy, utilizing the immune system to fight cancer, is an evolving strategy.
Lactobacillus reuteri, a gut bacterium, possesses immunomodulatory properties beneficial for reducing inflammation.

Purpose of the Study:

To develop a bifunctional mRNA delivery system for enhanced cancer immunotherapy.
To investigate the potential of Lactobacillus reuteri lysate as a component of mRNA delivery systems.
To evaluate the efficacy of the developed system in preclinical cancer models.

Main Methods:

Preparation of a bifunctional mRNA delivery system, DMP-Lac, codelivering Lactobacillus reuteri lysate and IL-23A mRNA.
Assessment of mRNA delivery efficiency and immune system activation in vivo.
Evaluation of therapeutic effects in CT26 abdominal and lung metastasis models.
Utilizing protein chip technology to elucidate the mechanism of action.

Main Results:

The DMP-Lac/IL-23A system achieved a high mRNA delivery efficiency of 75.56% ± 0.85%.
The system effectively promoted in vivo immune system maturation and activation.
Significant therapeutic effects were observed in metastasis models, with a tumor inhibition rate of 97.92% in the DMP-Lac/IL-23A group.
Lactobacillus reuteri lysate acted as an immune adjuvant, modulating immune cells, while IL-23 mRNA influenced downstream factors.

Conclusions:

The developed bifunctional mRNA formulation, DMP-Lac/IL-23A, functions as a tumor-specific nanomedicine.
This system demonstrates potent anticancer immunotherapeutic effects.
It offers an advanced strategy for colon cancer immunogene therapy by combining targeted mRNA delivery with immune stimulation.