Construction of an endoplasmic reticulum stress and cuproptosis -related lncRNAs signature in chemosensitivity in hepatocellular carcinoma by comprehensive bioinformatics analysis
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View abstract on PubMed
Summary
This summary is machine-generated.This study identifies a four-lncRNA signature linked to endoplasmic reticulum stress and cuproptosis in hepatocellular carcinoma (HCC). This signature accurately predicts patient prognosis and clinical outcomes in HCC.
Area Of Science
- Oncology
- Molecular Biology
- Bioinformatics
Background
- Hepatocellular carcinoma (HCC) prognosis is significantly impacted by endoplasmic reticulum stress (ERS) and cuproptosis.
- Identifying reliable prognostic markers is crucial for improving HCC patient outcomes.
Purpose Of The Study
- To develop a prognostic signature for HCC based on ERS and cuproptosis-related long non-coding RNAs (lncRNAs).
- To evaluate the predictive value of this signature for patient survival and clinical classification.
Main Methods
- Utilized TCGA database for clinical and transcriptomic data of HCC patients.
- Applied correlation tests, Cox regression, and LASSO for signature development.
- Validated prognostic value using Kaplan-Meier, ROC curves, and nomograms.
- Assessed immune and mutational status (TMB, ESTIMATE) and performed in vitro experiments (qRT-PCR, CCK-8).
Main Results
- A four-lncRNA signature (NRAV, SNHG3, LINC00839, AC004687.1) was identified.
- High-risk groups showed poorer prognosis, with the signature acting as an independent prognostic indicator.
- The signature correlated with clinicopathological features and predicted immune status and TMB.
- Enrichment analysis indicated involvement of biological membrane pathways.
Conclusions
- The developed ERS and cuproptosis-related lncRNA signature is a robust predictor of HCC survival.
- This signature can aid in clinical classification and predicting treatment efficacy for HCC patients.
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