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Hygroscopic bioactive light-cured composite promoting dentine bridge formation.
Yunzi Long1, Guibin Huang2, Siyi Liu2
1Department of General Dentistry II, Peking University School and Hospital of Stomatology, Beijing 100081, PR China.
Regenerative Biomaterials
|October 14, 2024
Summary
A new hygroscopic light-cured bioactive composite (LC-BG) enhances water absorption for improved bioactivity in direct pulp capping. This material demonstrated superior calcified bridge integrity and odontogenic gene expression compared to TheraCal LC.
Area of Science:
- Biomaterials Science
- Dental Materials Science
- Regenerative Dentistry
Background:
- TheraCal LC, a light-cured bioactive composite, shows limitations in bioactivity due to poor water absorption, hindering its efficacy in direct pulp capping (DPC).
- Enhancing water absorption in resin-based bioactive materials is crucial for optimizing the biomineralization process in light-cured materials.
Purpose of the Study:
- To develop and evaluate a novel hygroscopic, light-cured bioactive composite (LC-BG) for improved direct pulp capping applications.
- To assess the bioactivity, biocompatibility, and regenerative potential of the new composite compared to a commercial control.
Main Methods:
- A hygroscopic composite was constructed using bioactive glass (BG) encapsulated in poly (ethylene glycol) (PEG) within a resin matrix.
- Ion release, ion exchange, surface morphology (SEM), shear bond strength, cytotoxicity (CCK-8), gene expression (RT-PCR), and DPC assays were performed.
- In vivo evaluation assessed calcified bridge formation, cell differentiation markers (DSPP, DMP-1), inflammatory cell infiltration, and cytokine expression (IL-1β, TNF-α).
Main Results:
- The developed light-cured BG (LC-BG) composite exhibited faster ion release and exchange than TheraCal LC, with observed macropores and hydroxyapatite coatings.
- LC-BG demonstrated non-toxicity, upregulated odontogenic gene expression, and comparable shear bond strength to TheraCal LC.
- In vivo, LC-BG showed significantly better calcified bridge integrity and increased DSPP/DMP-1 positive cells, with reduced expression of inflammatory cytokines compared to TheraCal LC.
Conclusions:
- The novel hygroscopic LC-BG composite exhibits enhanced bioactivity and promotes odontogenic differentiation compared to TheraCal LC.
- LC-BG demonstrates superior performance in inducing calcified bridge formation in direct pulp capping procedures.
- This enhanced hygroscopic composite holds significant promise for improving regenerative outcomes in dental pulp capping therapies.
