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  11. Effective Patchiness From Critical Points Of A Coarse-grained Protein Model With Explicit Shape And Charge Anisotropy

Effective patchiness from critical points of a coarse-grained protein model with explicit shape and charge anisotropy

Jens Weimar1, Frank Hirschmann1, Martin Oettel1

  • 1Institute for Applied Physics, University of Tübingen, Auf der Morgenstelle 10, 72076 Tübingen, Germany. martin.oettel@uni-tuebingen.de.

Soft Matter
|October 14, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Colloidal theory helps understand protein solutions, but anisotropic interactions require careful application of stability criteria. This study models bovine serum albumin (BSA) and finds charge anisotropy significantly impacts phase behavior.

Area of Science:

  • Colloid and soft matter physics
  • Computational biophysics
  • Protein science

Background:

  • Colloidal models explain protein solution behavior like aggregation and phase transitions.
  • Isotropic interaction models and the Noro-Frenkel rule are common for assessing solution stability.
  • Anisotropic protein interactions, like those in patchy particle models, alter stability criteria.

Purpose of the Study:

  • To investigate the effects of charge anisotropy on the phase diagram of a coarse-grained bovine serum albumin (BSA) model.
  • To develop methods for assigning effective patchiness to anisotropic protein models by comparing critical properties to the Kern-Frenkel model.
  • To assess the applicability of existing colloidal theory criteria to shape-anisotropic models.

Main Methods:

  • Utilized low-resolution, coarse-grained simulations to model bovine serum albumin (BSA).

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  • Determined the phase diagram of the BSA model at its isoelectric point.
  • Compared critical properties of the BSA model to the Kern-Frenkel (KF) patchy particle model to assign effective patchiness.

    • Main Results:

    • Doubling the native charges on the BSA model increased its critical temperature (Tc) by approximately 14%.
    • The simulated BSA model exhibited critical properties comparable to a 3 to 5 patch Kern-Frenkel model.
    • Normalization of the critical point criterion becomes ambiguous for shape-anisotropic models.

    Conclusions:

    • Charge anisotropy significantly influences the phase behavior and critical temperature of protein solutions.
    • Existing colloidal theory criteria for solution stability should be applied cautiously to shape-anisotropic models.
    • Defining effective diameters for anisotropic models requires careful consideration of multiple physically plausible assignments.