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  11. Microfluidic-sers Sensing System Based On Dual Signal Amplification And Aptamer For Gastric Cancer Detection

Microfluidic-SERS sensing system based on dual signal amplification and aptamer for gastric cancer detection

Yong Huang1, Miao Chen2, Fengjuan Jiang3

  • 1Department of Gastrointestinal Surgery, Jiangdu People's Hospital Affiliated to Yangzhou University, Yangzhou, 225200, P. R. China.

Mikrochimica Acta
|October 14, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

This study presents a novel microfluidic sensor using surface-enhanced Raman scattering (SERS) to detect matrix metalloproteinase-9 (MMP-9) and interleukin-6 (IL-6) for early gastric cancer screening.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Matrix metalloproteinase-9 (MMP-9) and interleukin-6 (IL-6) are key biomarkers in tumorigenesis.
  • Accurate and sensitive detection of these biomarkers is crucial for early cancer diagnosis.

Purpose of the Study:

  • To develop an efficient microfluidic-SERS sensing system for high-sensitivity and specificity detection of MMP-9 and IL-6.
  • To investigate the potential of this system for early gastric cancer screening.

Main Methods:

  • Fabrication of a microfluidic-SERS sensing system using gold-coated iron tetroxide particles (Fe3O4@AuNPs) and gold nanocages (AuNCs).
  • Utilized an aptamer recognition-release mechanism and a dual signal amplification strategy.
  • Incorporated magnetic enrichment using Fe3O4@AuNPs on a microfluidic chip.
Keywords:
AptamerBiomarkerGastric cancerMicrofluidic chip

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Main Results:

  • Achieved dual signal amplification of SERS, leading to enhanced sensitivity.
  • Demonstrated high specificity due to aptamer-target affinity.
  • Obtained low limits of detection (LOD) of 0.178 pg/mL for MMP-9 and 0.165 pg/mL for IL-6.

Conclusions:

  • The developed microfluidic-SERS system offers a sensitive and specific method for detecting MMP-9 and IL-6.
  • This sensing system shows significant promise for the early screening of gastric cancer.
SERS