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Related Experiment Video

Updated: Jun 10, 2025

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct

Heather J Clemons^1,2, Daniel J Hogan^1,2, Patrick O Brown^1,2

¹Department of Biochemistry, Stanford University School of Medicine, Palo Alto, California, United States of America.

|October 14, 2024

Summary

This summary is machine-generated.

Human fat cells (adipocytes) from different body areas are functionally distinct, specialized organs. Their unique gene expression patterns suggest depot-specific developmental programming, not just environmental responses.

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Last Updated: Jun 10, 2025

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

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Published on: May 19, 2023

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

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Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

Area of Science:

Human physiology
Developmental biology
Molecular genetics

Background:

Adipose tissue, comprising diverse anatomical depots, has largely unknown functional specializations.
The origin of depot-specific characteristics (intrinsic programming vs. environmental factors) remains unclear.

Purpose of the Study:

To investigate whether anatomically distinct human adipose depots exhibit unique functional characteristics.
To determine if depot-specific adipocyte traits arise from intrinsic developmental programs or microenvironmental influences.

Main Methods:

DNA microarrays were employed to compare mRNA expression in isolated human adipocytes and cultured adipose stem cells.
Analysis included cells from seven diverse anatomical depots, before and after ex vivo differentiation.

Main Results:

Adipocytes from different depots displayed distinct gene expression programs, mirroring anatomically related depots.
Depot-specific gene expression differences were observed and recapitulated in ex vivo differentiated progenitor cells.
Developmental transcription factors showed striking depot-specific expression patterns.

Conclusions:

Adipocytes from various anatomical depots are functionally distinct specialized organs.
Depot-specific adipocyte specialization reflects distinct, deterministically programmed developmental pathways.
These findings support a model of intrinsic programming for adipose tissue heterogeneity.