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Updated: Jun 10, 2025

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
Published on: March 20, 2019
PavSPLs are key regulators of growth, development, and stress response in sweet cherry
Xunju Liu1, Wanxia Sun1, Haobo Liu1
1Department of Plant Sciences, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang, Shanghai 200240, China.
Abstract:
SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes are plant-specific transcription factors essential for plant growth, development, and stress responses. Their roles in sweet cherry are not well understood. In this study, we identified and isolated 16 SPL genes from the sweet cherry genome, categorizing them into 5 subfamilies, with 12 PavSPLs predicted as miR156 targets. Promoter regions of PavSPLs contain cis-elements associated with light, stress, and phytohormone responses, indicating their role in biological processes and abiotic stress responses. Seasonal expression analysis showed that PavSPL regulates sweet cherry recovery after dormancy. Gibberellin (GA) treatment reduced PavSPL expression, indicating its role in GA-mediated processes. PavSPL14 overexpression in Arabidopsis thaliana resulted in earlier flowering and increased plant height and growth. Yeast two-hybrid assays showed an interaction between PavSPL14 and DELLA protein PavDWARF8, suggesting PavSPL14 and PavDWARF8 co-regulate growth and development. These findings lay the groundwork for further research on PavSPL function in sweet cherry.
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