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Related Experiment Videos

Gene amplification in human neuroblastomas: basic mechanisms and clinical implications.

G M Brodeur, R C Seeger

    Cancer Genetics and Cytogenetics
    |January 1, 1986
    PubMed
    Summary

    N-myc gene amplification, found in 38% of neuroblastomas, is linked to advanced disease stages and rapid progression. This amplification serves as a key prognostic factor in neuroblastoma.

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    Molecular analysis of gene amplification in tumors.

    Current protocols in human genetics·2008

    Area of Science:

    • Genetics
    • Oncology
    • Molecular Biology

    Background:

    • Double minutes (DM) and homogeneously staining regions (HSR) are chromosomal abnormalities common in neuroblastomas.
    • These abnormalities are frequently associated with amplified copies of the N-myc oncogene.

    Purpose of the Study:

    • To investigate the prevalence of N-myc gene amplification in primary neuroblastomas.
    • To determine the correlation between N-myc amplification and clinical parameters such as disease stage and progression.

    Main Methods:

    • Analysis of N-myc gene copy number in 89 primary neuroblastoma tumors.
    • Statistical analysis to correlate N-myc amplification with disease stage and progression.

    Main Results:

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  • N-myc amplification was identified in 38% (34 of 89) of neuroblastoma cases, with copy numbers ranging from 3- to 300-fold.
  • Amplification was predominantly observed in advanced disease stages (III and IV) (p < 0.01).
  • N-myc amplification strongly correlated with rapid tumor progression (p < 0.001).

    • Conclusions:

    • N-myc gene amplification is a significant prognostic factor in neuroblastoma.
    • Amplification of the N-myc oncogene plays a crucial role in the progression of neuroblastoma.