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Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

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Related Experiment Video

Updated: Jun 27, 2026

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Published on: September 17, 2011

Unique Glycans in Synaptic Glycoproteins in Mouse Brain.

Maxence Noel¹, Suttipong Suttapitugsakul¹, Mohui Wei¹

¹Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, United States.

ACS Chemical Neuroscience

|October 14, 2024

Summary

Researchers identified novel galactosylated glycoproteins in the brain, crucial for neuronal function and synaptic development. This discovery sheds light on the unique glycosylation patterns essential for brain activity.

Keywords:

ST6Gal1 biotinylated CMP-sialic acid brain click-chemistry galactosylated glycoproteins glycoproteomics

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Related Experiment Videos

Last Updated: Jun 27, 2026

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Published on: September 17, 2011

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

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Published on: January 29, 2019

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Published on: March 11, 2020

Area of Science:

Neuroscience
Glycobiology
Biochemistry

Background:

Synaptic function relies on glycoproteins, but their carbohydrate modifications are poorly understood.
Galactose is vital for brain function and development, yet its addition to N-glycans in the brain is restricted.

Purpose of the Study:

To explore novel glycosylation patterns in the brain.
To identify proteins undergoing galactosylation and their synaptic localization.

Main Methods:

Utilized an orthogonal technology involving a biotinylated sialic acid derivative (CMP-biotin-Sia) and recombinant sialyltransferase (rST6Gal1).
Applied immunohistochemical analysis and glycoproteomic analyses to identify and localize labeled glycoproteins.

Main Results:

Identified galactosylated N-glycoproteins primarily located in pre- and postsynaptic membranes.
Glycoproteomic analysis revealed these glycoproteins are involved in synaptic function, cell adhesion, and extracellular matrix interactions.

Conclusions:

Discovered previously unknown galactosylated N-glycoproteins in the brain.
These findings provide new insights into the specific and unusual nature of protein glycosylation within synapses.