Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Placental plasminogen activator inhibitor 1 is induced by platelet-derived TGF-β independently of TGF-β receptor 3 and is upregulated in preeclampsia.

Molecular human reproduction·2026

Same author

ROS1-Mutated Inoperable Spindle Cell Sarcoma Treated With Repotrectinib-A Case Report.

JCO precision oncology·2026

Same author

A novel algae oil-based emulsion enhances chemotherapy outcome in human cancer models.

Prostaglandins, leukotrienes, and essential fatty acids·2026

Same author

Nationwide treatment patterns and outcomes of localised soft tissue sarcoma in Austria: real-world data from the Austrian Sarcoma Registry.

Journal of orthopaedic surgery and research·2026

Same author

Bacterial cell fate under stress: lipid remodeling and antimicrobial peptide attack.

npj antimicrobials and resistance·2026

Same author

Impact of Test Set Composition on AI Performance for Pediatric Radiograph Appendicular Skeleton Fracture Detection.

Radiology·2026

Search research articles

Related Experiment Video

Updated: Jul 16, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

A human ex vivo skin model breaking boundaries.

Astrid Wurbs¹, Christina Karner¹, Djenana Vejzovic¹

¹Division of Biomedical Research, Core Facility Alternative Biomodels and Preclinical Imaging, Medical University of Graz, Roseggerweg 48, 8036, Graz, Austria.

Scientific Reports

|October 14, 2024

Summary

This study shows that human skin models can be cultured at room temperature without a CO2 incubator. This novel method maintains tissue integrity and immune cell viability for three days, enabling new research possibilities.

Keywords:

Ex vivo skin model Extracellular vesicles Juvenile foreskin Room temperature

More Related Videos

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

Related Experiment Videos

Last Updated: Jul 16, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

Area of Science:

Biotechnology
Dermatology
Cell Biology

Background:

Ex vivo human skin models are crucial for skin research, offering physiological relevance.
Traditional cultivation requires controlled environments (37°C, CO2 incubator), limiting assessment of real-world atmospheric variations.
Current methods restrict the flexibility needed to study skin's response to diverse environmental exposures.

Purpose of the Study:

To develop and validate a novel method for culturing ex vivo human skin models outside of a CO2 incubator.
To assess the stability and resilience of the skin model under room temperature conditions.
To enable comprehensive analysis of cellular responses, including extracellular vesicles, to various stimuli.

Main Methods:

Utilized a foreskin-derived human skin model cultured at room temperature.
Employed a CO2-independent, serum-free media for cultivation.
Cultivated the model for three days, monitoring tissue integrity and immune cell preservation.
Stimulated the model with cytotoxic and inflammatory agents and analyzed supernatant for proteins and extracellular vesicles.

Main Results:

Successfully cultured the human skin model at room temperature, outside a CO2 incubator.
Maintained tissue integrity and immune cell viability over a three-day cultivation period.
Demonstrated that the model responds to cytotoxic and inflammatory stimuli with analyzable supernatant components, including extracellular vesicles.

Conclusions:

The developed method allows for successful ex vivo human skin model cultivation at room temperature.
This approach preserves tissue and immune cell integrity, offering a stable and resilient model.
The model is suitable for studying skin responses to various stimuli, providing comprehensive cellular communication insights via supernatant analysis.