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Updated: Jun 10, 2025
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Published on: April 3, 2016
Proteomic analysis of CD29+ Müller cells reveals metabolic reprogramming in rabbit myopia model
Chae-Eun Moon1, Jun-Ki Lee1, Hyunjin Kim1
1Department of Ophthalmology, Institute of Vision Research, Severance Hospital, Yonsei University College of Medicine, 50 Yonsei‑ro, Seodaemun‑gu, Seoul, 03722, Republic of Korea.
Abstract:
The prevalence of myopia is rapidly increasing, significantly impacting the quality of life of affected individuals. Prior research by our group revealed reactive gliosis in Müller cells within myopic retina, prompting further investigation of their role in myopia, which remains unclear. In this study, we analyzed protein expression changes in CD29+ Müller cells isolated from a form deprivation-induced rabbit model of myopia using magnetic activated cell sorting to investigate the role of these cells in myopia. As the principal glial cells in the retina, Müller cells exhibited significant alterations in the components of metabolic pathways, particularly glycolysis and angiogenesis, including the upregulation of glycolytic enzymes, such as lactate dehydrogenase A and pyruvate kinase, implicated in the adaptation to increased metabolic demands under myopic stress. Additionally, a decrease in the expression of proteins associated with oxygen transport suggested enhanced vulnerability to oxidative stress. These findings highlight the proactive role of CD29+ Müller cells in modifying the retinal environment in response to myopic stress and provide valuable insights into mechanisms that could help mitigate myopia progression.
Insights
Myopic stress alters Müller cells, the retina's main glial cells. These cells adapt their metabolism, potentially increasing vulnerability to oxidative stress and offering insights into myopia progression.
Area of Science:
- Ophthalmology
- Cell Biology
- Neuroscience
Background:
- Myopia prevalence is rising globally, impacting quality of life.
- Müller cells, the retina's principal glial cells, show reactive gliosis in myopia.
- The specific role of Müller cells in myopia pathogenesis is not fully understood.
Purpose of the Study:
- To investigate the protein expression changes in CD29+ Müller cells from a rabbit model of myopia.
- To elucidate the role of Müller cells in adapting to myopic stress.
Main Methods:
- Used a form deprivation-induced rabbit model of myopia.
- Isolated CD29+ Müller cells using magnetic activated cell sorting.
- Analyzed protein expression changes via mass spectrometry.
Main Results:
- Müller cells showed significant alterations in metabolic pathways, including glycolysis and angiogenesis.
- Upregulation of glycolytic enzymes (lactate dehydrogenase A, pyruvate kinase) was observed.
- Decreased expression of oxygen transport proteins indicated increased vulnerability to oxidative stress.
Conclusions:
- CD29+ Müller cells play a proactive role in modifying the retinal environment during myopic stress.
- These cellular adaptations offer potential targets for mitigating myopia progression.
- Understanding Müller cell responses provides insights into myopia mechanisms.
