A Cyclin Gene OsCYCB1;5 Regulates Seed Callus Induction in Rice Revealed by Genome Wide Association Study
Wenjing Song1, Jian Zhang1, Wenyu Lu1
1National Engineering Research Center of Plant Aerospace-Mutation Breeding, South China Agricultural University, Guangzhou, 510642, China.
Rice (New York, N.Y.)
|October 14, 2024
Summary
This study identified 104 significant genetic loci and 13 candidate genes influencing rice callus induction rate. Knockout mutants of OsCycB1;5 confirmed its crucial role in callus formation, aiding plant genetic improvement.
Area of Science:
- Plant Science
- Genomics
- Biotechnology
Background:
- Plant tissue culture is vital for functional genomics and crop breeding.
- Callus induction ability is essential for Agrobacterium-mediated genetic transformation.
Purpose of the Study:
- To identify genetic loci and candidate genes controlling callus induction rate (CIR) in rice.
- To validate the role of candidate genes in rice callus formation.
Main Methods:
- Genome-wide association study (GWAS) on 368 rice accessions.
- Gene function annotation and transcriptome analysis.
- CRISPR/Cas9 gene editing to create OsCycB1;5 knockout mutants.
Main Results:
- Identified 104 significant single nucleotide polymorphism (SNP) loci associated with CIR.
- Discovered 13 high-confidence candidate genes, including those related to auxin, CYC cyclins, and histone methylation.
- OsCycB1;5 knockout mutants showed significantly reduced callus induction and proliferation.
Conclusions:
- OsCycB1;5 is a key gene required for efficient callus formation in rice.
- Identified loci and genes provide valuable targets for improving rice genetic transformation and breeding.
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