Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

52.4K
Overview
52.4K
Bacterial Signaling01:30

Bacterial Signaling

31.6K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
31.6K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

2
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
2
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

2
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
2
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

1
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
1

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trimerization of CD40L-specific affibody molecules using collagen domains enhances target binding and CD40 blockade.

Cellular and molecular life sciences : CMLS·2026
Same author

Combining bacterial display and protein language models to engineer a CD69-binding affibody for molecular imaging of immune activation.

Protein engineering, design & selection : PEDS·2026
Same author

Engineering a conditionally active cetuximab prodrug via affibody-based paratope masking.

Journal of biological engineering·2026
Same author

Engineering affibody domains as anti-idiotypic masks for nivolumab-based prodrugs.

Protein engineering, design & selection : PEDS·2026
Same author

Drug Development.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Affibody-Derived Drug Conjugates Targeting The Epidermal Growth Factor Receptor Are Potent And Specific Cytotoxic Agents.

ACS pharmacology & translational science·2025
Same journal

Coumarins Disrupt Cell-Cell Communication and Virulence in Priority Pathogens: Targeting the PQS Signalling System in Pseudomonas aeruginosa.

Microbial biotechnology·2026
Same journal

Engineering Pseudomonas protegens as a Targeted Antifungal-Effector Delivery Chassis via Heterologous Type III Secretion System Reconstitution.

Microbial biotechnology·2026
Same journal

Adaptive Laboratory Evolution Unlocks Membrane Permeability as a Key Limitation in Long-Chain Alcohol Metabolism by Pseudomonas putida KT2440.

Microbial biotechnology·2026
Same journal

Correction to 'Unveiling the Potential of Lentilactobacillus hilgardii in Malolactic Fermentation: Comparative Genomics and Fermentation Dynamics'.

Microbial biotechnology·2026
Same journal

NADPH Dehydrogenase Gene Regulates Energy Distribution and Fatty Acid Metabolism During Fruiting Body Formation in the Filamentous Fungus Podospora anserina.

Microbial biotechnology·2026
Same journal

Promoting Microbiology Literacy and Holistic Health: IMiLI and IMiLI-SAC Perspectives on Microbes, Diet, Lifestyle and Society-A 5-Year Journey.

Microbial biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jun 10, 2025

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
15:28

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Published on: November 16, 2012

14.5K

Surface-engineered bacteria in drug development.

Charles Dahlsson Leitao1, Stefan Ståhl1, John Löfblom1

  • 1Department of Protein Science, KTH Royal Institute of Technology, Stockholm, Sweden.

Microbial Biotechnology
|October 15, 2024
PubMed
Summary
This summary is machine-generated.

Bacterial surface display with fluorescence-activated cell sorting offers a robust method for generating therapeutic proteins, presenting unique drug development possibilities. This system aids in creating affibody molecules for treating diseases like cancer.

More Related Videos

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

8.9K
Isolation of Soil Microorganisms Using iChip Technology
05:33

Isolation of Soil Microorganisms Using iChip Technology

Published on: January 10, 2025

813

Related Experiment Videos

Last Updated: Jun 10, 2025

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
15:28

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Published on: November 16, 2012

14.5K
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

8.9K
Isolation of Soil Microorganisms Using iChip Technology
05:33

Isolation of Soil Microorganisms Using iChip Technology

Published on: January 10, 2025

813

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Protein Engineering

Background:

  • Phage display is a common method for generating affinity proteins.
  • Therapeutic protein development requires robust and versatile systems.
  • Bacterial surface display offers an alternative to traditional methods.

Purpose of the Study:

  • To highlight bacterial surface display combined with fluorescence-activated cell sorting (FACS) as a powerful system for generating therapeutic affinity proteins.
  • To present this system as a viable alternative to phage display.
  • To showcase its applications in drug development, including preclinical evaluation of affibody molecules.

Main Methods:

  • Utilizing bacterial surface display technology.
  • Employing fluorescence-activated cell sorting (FACS) for real-time monitoring and sorting of cell populations.
  • Generating and evaluating affibody molecules for therapeutic and diagnostic purposes.

Main Results:

  • Bacterial surface display combined with FACS is a versatile and robust system.
  • The system enables real-time monitoring and sorting, offering unique drug development possibilities.
  • Several affibody molecules have been developed using this system and are undergoing preclinical evaluation for cancer and neurodegenerative diseases.

Conclusions:

  • Bacterial surface display with FACS is a powerful alternative to phage display for affinity protein generation.
  • This system has significant potential in drug development, including the creation of targeted therapies.
  • Further applications include epitope mapping and enzyme specificity evolution.