Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

11.2K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Screening the Tox21 Compound Library for Chemicals That Stimulate the Adrenergic β1 Receptor.

Chemical research in toxicology·2026
Same author

Expanded Tox21 Biological Assay Panel for the Prediction of Drug-Induced Liver Injury and Cardiotoxicity.

Environmental health perspectives·2026
Same author

Quantitative high-throughput screening identifies small-molecule modulators of Wnt/β-catenin signaling.

Frontiers in pharmacology·2026
Same author

Impact of Chemical Quality on High-Throughput in Vitro Assays: A Tox21 Study.

Chemical research in toxicology·2026
Same author

Identification of potential autophagy inducers through high-throughput and high-content imaging analysis.

Molecular pharmacology·2026
Same author

Retinoid-X receptor action and disruption in development.

Developmental biology·2026

Related Experiment Video

Updated: Jun 10, 2025

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
06:07

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Published on: June 7, 2020

4.8K

Using Reporter Gene Assays to Screen and Identify Chemical Compounds that Modulate Estrogen Receptor Activity.

Masato Ooka1, Srilatha Sakamuru1, J David Furlow2

  • 1National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland.

Current Protocols
|October 15, 2024
PubMed
Summary

High-throughput screening methods were developed to rapidly test compounds modulating estrogen receptor alpha (ERα). These assays enable efficient identification and confirmation of ERα modulators for pharmaceutical applications.

Keywords:
estrogen receptor alphaestrogen receptor reporter gene assayquantitative high throughput screening

More Related Videos

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
08:00

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

Published on: December 4, 2016

7.5K
Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
14:13

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Published on: January 1, 2018

13.1K

Related Experiment Videos

Last Updated: Jun 10, 2025

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
06:07

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Published on: June 7, 2020

4.8K
Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
08:00

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

Published on: December 4, 2016

7.5K
Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
14:13

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Published on: January 1, 2018

13.1K

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Drug Discovery

Background:

  • Estrogen receptor alpha (ERα) is crucial for reproduction and homeostasis, with its disruption linked to diseases like cancer.
  • Identifying ERα modulators is vital for pharmaceutical development and understanding endocrine disruption.
  • High-throughput screening (HTS) is essential for rapidly testing large compound libraries.

Purpose of the Study:

  • To establish and describe robust HTS methods for identifying ERα modulators.
  • To detail protocols for cell-based assays using reporter gene systems.
  • To enable efficient screening of tens of thousands of compounds.

Main Methods:

  • Utilized two distinct cell lines: MCF7-VM7Luc4E2 (luminescence readout) and HEK293-ERα-bla (fluorescence readout).
  • Developed protocols for cell plating, treatment, and data analysis in 384-well and 1536-well formats.
  • Implemented a two-tiered approach: initial screening followed by orthogonal confirmation.

Main Results:

  • Successfully established HTS assays for ERα modulator identification.
  • Demonstrated the utility of both luminescence and fluorescence-based reporter systems.
  • Validated the methods for identifying and confirming ERα activators and inhibitors.

Conclusions:

  • The described HTS methods provide an efficient platform for discovering ERα modulators.
  • These assays are valuable tools for drug discovery and research into ERα function.
  • The protocols facilitate rapid assessment of compounds for endocrine-disrupting potential or therapeutic use.