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Related Experiment Video

Updated: Jun 10, 2025

In vitro Uncoating of HIV-1 Cores

In vitro Uncoating of HIV-1 Cores

Published on: November 8, 2011

HIV-1 assembly - when virology meets biophysics.

Claire Lacouture¹, Baptiste Carrio¹, Cyril Favard¹

¹Membrane Domains and Viral Assembly, Institut de Recherche en Infectiologie de Montpellier (IRIM), UMR 9004, CNRS, Montpellier University, 1919, route de Mende, 34293 Montpellier CEDEX 5, France.

Journal of Cell Science

|October 15, 2024

Summary

Human immunodeficiency virus type 1 (HIV-1) Gag protein drives viral particle budding by self-assembling and bending host cell membranes. This process hijacks cellular machinery, but whether Gag

Keywords:

Cortical actin Gag HIV-1 Membrane curvature Phospholipids

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Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes

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Published on: February 22, 2017

Area of Science:

Virology
Cell Biology
Biophysics

Background:

Cells produce vesicles via lipid membranes and molecular machinery.
Enveloped RNA viruses like HIV-1 hijack cellular pathways for particle budding.
HIV-1 budding primarily involves the self-assembly of the structural Gag protein.

Purpose of the Study:

To review the mechanisms of HIV-1 particle assembly and budding.
To explore the role of Gag self-assembly in viral particle formation.
To investigate Gag's interaction with host cell lipids and proteins.

Main Methods:

Review of virology literature.
Incorporation of quantitative biophysics studies on viral self-assembly.
Analysis of Gag's interaction with host cell plasma membrane lipids and proteins.

Main Results:

HIV-1 Gag binds and segregates host plasma membrane lipids during self-assembly.
Gag self-assembly on genomic RNA and plasma membrane induces membrane bending.
Host cell proteins, including cortical actin factors, likely assist in particle formation.

Conclusions:

Gag's self-assembly is a key driver of HIV-1 particle budding.
Gag reorganizes the plasma membrane and utilizes host factors for assembly.
Further research is needed to confirm if Gag's assembly energy alone is sufficient for budding.