Tauroursodeoxycholic acid combined with selenium accelerates bone regeneration in ovariectomized rats

Area of Science:

• Biomedical Science
• Cell Biology
• Bone Biology

Background:

• Osteoporosis impairs bone repair, often linked to oxidative stress.
• Antioxidants show promise in treating osteoporosis by mitigating oxidative stress.
• Tauroursodeoxycholic acid (TUDCA) and Selenium (Se) possess known antioxidant properties and bone regenerative potential.

Purpose of the Study:

• To evaluate the protective effects of combined Se and TUDCA on bone regeneration in osteoporotic models.
• To investigate the underlying mechanisms of Se/TUDCA in promoting bone repair.
• To compare the efficacy of combined Se/TUDCA with individual Se or TUDCA treatments.

Main Methods:

• MC3T3-E1 cells were treated with H2H2, Se, TUDCA, and Se/TUDCA to assess matrix mineralization and oxidative stress markers.
• Intracellular and mitochondrial reactive oxygen species (ROS) levels were measured.
• Silent information regulator type 1 (SIRT1) expression was analyzed.
• An ovariectomized (OVX) rat model was used to evaluate bone repair efficacy in vivo.

Main Results:

• Se, TUDCA, and Se/TUDCA treatments increased matrix mineralization and anti-oxidative stress marker expression in H2H2-treated MC3T3-E1 cells.
• All treatments reduced intracellular and mitochondrial ROS levels.
• SIRT1 expression was upregulated by Se, TUDCA, and Se/TUDCA in H2H2-treated cells.
• Se/TUDCA demonstrated superior efficacy in promoting bone repair in the OVX rat model compared to Se or TUDCA alone.

Conclusions:

• Combined Se and TUDCA therapy effectively stimulates bone regeneration by inhibiting oxidative stress.
• Se/TUDCA exhibits enhanced efficacy over individual components in both cellular and animal studies.
• This combination represents a promising therapeutic strategy for enhancing bone repair in osteoporosis.