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Related Experiment Video

Updated: Jun 10, 2025

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
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Modulating membrane-bound enzyme activity with chemical stimuli.

Zhilan Zhou1, Zening Huang2, Yiyuan Tang3

  • 1Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China; Zhejiang Cancer Hospital, The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, 310022, China.

European Journal of Medicinal Chemistry
|October 15, 2024
PubMed
Summary

This review covers chemical stimuli that control membrane-bound enzyme activity for cellular homeostasis and disease treatment. Understanding these enzyme regulations is key for developing new therapies.

Keywords:
Chemical stimuliDrug targetMembrane-bound enzymeModulating enzyme activityModulation mechanisms

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Area of Science:

  • Biochemistry and Molecular Biology
  • Cellular Biology
  • Pharmacology

Background:

  • Membrane-bound enzymes are crucial for cellular processes, homeostasis, and signaling.
  • Dysregulation of these enzymes is linked to various diseases.
  • Targeted modulation of enzyme activity presents therapeutic opportunities.

Purpose of the Study:

  • To review chemical stimuli-responsive strategies for modulating membrane-bound enzyme activity.
  • To explore diverse stimuli (small molecules, proteins, nucleic acids, bifunctional molecules) for enzyme modulation.
  • To discuss mechanisms, binding modalities, and medical applications of enzyme activity regulation.

Main Methods:

  • Systematic review of literature on chemical stimuli-responsive strategies.
  • Delineation of enzyme activity regulation mechanisms (e.g., substrate blockade, conformational changes).
  • Analysis of binding interactions between stimuli and enzymes.

Main Results:

  • Identified diverse chemical stimuli capable of inhibiting or enhancing enzyme function.
  • Elucidated key mechanisms controlling enzyme activity via chemical modulation.
  • Highlighted potential medical applications and future research directions.

Conclusions:

  • Chemical stimuli offer powerful tools for regulating membrane-bound enzyme activity.
  • Understanding these interactions is vital for advancing biomedical research.
  • Targeted enzyme modulation holds promise for novel therapeutic interventions.