Systematic preparation of animal-derived glycosaminoglycans: Research progress and industrial significance
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Updated: Jun 10, 2025

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
Sinong Liu1, Yanpeng Xiang1, Chang Xu2
1College of Food Science, Shenyang Agricultural University, Shenyang 110866, PR China.
Abstract:
Impurities and isomerized polysaccharides affect the analytical accuracy of glycosaminoglycans (GAGs) structure and bioactivity, hindering their application in food and medicine. Preparing homogeneous GAGs components is essential for exploring structure-potency relationships and facilitating industrial production. This review primarily summarizes research on animal-derived GAGs preparation over the past five years, standardizing the preparation process into four operational units: pre-extraction treatment, extraction of crude polysaccharides, refinement of crude polysaccharides, and separation of GAGs components. Analyzed for scientific research and industrial production, the principles and application conditions of traditional means and novel techniques to preparing GAGs are comprehensively emphasized, exploring the effects of different treatments on biological activity and structure. Current challenges and development trends are illuminated. This review aims to lay a foundation for the in-depth study of GAGs structure, bioactivity, and function, providing theoretical references for the comprehensive utilization of animal raw materials and the development of animal polysaccharide deep-processing industries.
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