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Related Experiment Video

Updated: Jun 10, 2025

Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry

Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry

Published on: January 28, 2017

Embryonic macrophages support endocrine commitment during human pancreatic differentiation.

Adriana Migliorini¹, Sabrina Ge², Michael H Atkins³

¹McEwen Stem Cell Institute, University Health Network, Toronto, ON M5G 1L7, Canada.

|October 15, 2024

Summary

This summary is machine-generated.

Macrophages are crucial for pancreatic endocrine cell development and survival. This study reveals their supportive role in vitro, suggesting potential applications in tissue engineering for diabetes treatment.

Keywords:

beta cells human pancreatic development human pluripotent stem cells immune cells islets organoids regenerative medicine single-nucleus RNA sequencing tissue remodeling tissue-resident macrophages

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Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

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Published on: June 2, 2018

Area of Science:

Developmental biology
Immunology
Endocrinology

Background:

Pancreatic endocrine cell development involves complex interactions within the tissue microenvironment.
Hematopoietic cells, including macrophages, are present in the developing human pancreas, but their specific role in endocrinogenesis is unclear.

Purpose of the Study:

To investigate the role of hematopoietic cells, particularly macrophages, in human pancreatic endocrine development.
To model the interaction between macrophages and endocrine cells in vitro.

Main Methods:

Single-nucleus RNA sequencing (snRNA-seq) of second-trimester human pancreas to identify hematopoietic cell populations.
Development of a co-culture system using human embryonic stem cell-derived endocrine and macrophage organoids.

Main Results:

snRNA-seq identified diverse hematopoietic cells, including two distinct macrophage subsets, in the developing human pancreas.
Co-culture experiments demonstrated that macrophages support the differentiation and viability of endocrine cells in vitro.
Macrophages enhanced tissue engraftment in the organoid model.

Conclusions:

Tissue-resident macrophages play a supportive role in human pancreatic endocrine cell development and survival.
These findings highlight the potential of macrophages in tissue engineering strategies for diabetes.
Macrophage-endocrine cell interactions are critical for pancreatic organogenesis.