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The subcortical maternal complex modulates the cell cycle during early mammalian embryogenesis via 14-3-3
Zhuo Han1, Rui Wang2,3,4, Pengliang Chi1
1Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China.
Nature Communications
|October 15, 2024
Summary
The subcortical maternal complex (SCMC) safeguards fertility by stabilizing 14-3-3 protein. This mechanism ensures proper cell cycle control and mitotic entry in early mammalian embryos, crucial for embryogenesis.
Area of Science:
- Reproductive Biology
- Cellular and Molecular Biology
- Developmental Biology
Background:
- The subcortical maternal complex (SCMC) is vital for mammalian female fertility.
- Its precise role in early embryonic development and cell cycle regulation is not fully understood.
Purpose of the Study:
- To elucidate the molecular mechanism by which the SCMC regulates the cell cycle in early embryos.
- To identify key protein interactions within the SCMC.
Main Methods:
- Structural analysis of the SCMC complex.
- Investigating protein-protein interactions and phosphorylation events.
- Studying SCMC function in mouse and human oocytes/embryos.
Main Results:
- Identified 14-3-3 protein as a crucial component of the SCMC.
- Discovered that TLE6 phosphorylation recruits 14-3-3 to the SCMC.
- Demonstrated SCMC-mediated stabilization of 14-3-3 and regulation of CDC25B for cell cycle progression.
- Confirmed conserved SCMC-14-3-3-CDC25B interactions in human embryos.
Conclusions:
- The SCMC plays a critical role in safeguarding female fertility through cell cycle control in early embryogenesis.
- The SCMC stabilizes 14-3-3, regulating CDC25B and ensuring proper mitotic entry in zygotes.
- This mechanism is conserved across mammalian species, including humans.
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