Detection of Thrombosis Using Soluble C-Type Lectin-like Receptor-2 with D-Dimer Level and Platelet Count

Area of Science:

• Biochemistry
• Hematology
• Medical Diagnostics

Background:

• Soluble C-type lectin-like receptor -2 (sCLEC-2) is a recognized marker of platelet activation.
• Existing research suggests potential for formulas combining sCLEC-2 with platelet count and D-dimer levels for disease detection.
• Thrombotic diseases, including microvascular thrombosis (MVT), arterial thromboembolism (ATE), and venous thromboembolism (VTE), pose significant health risks.

Purpose of the Study:

• To evaluate the diagnostic capability of sCLEC-2 levels, alone and in combination formulas, for various thrombotic diseases.
• To assess the utility of sCLEC-2/platelet count (sCLEC-2/PLT), sCLEC-2 × D-dimer (sCLEC-2xDD), and sCLEC-2xDD/PLT ratios in diagnosing MVT, ATE, and VTE.
• To determine the effectiveness of these parameters in differentiating thrombotic conditions from non-thrombotic states and infections.

Main Methods:

• Measurement of plasma sCLEC-2 levels in patients with and without thrombotic or infectious diseases.
• Calculation and analysis of sCLEC-2/PLT, sCLEC-2xDD, and sCLEC-2xDD/PLT ratios.
• Utilized receiver operating characteristic (ROC) analysis for diagnostic performance evaluation.

Main Results:

• Plasma sCLEC-2 levels were elevated in patients with infectious or thrombotic diseases compared to healthy controls.
• The sCLEC-2/PLT ratio was highest in MVT patients; sCLEC-2xDD was elevated in MVT and VTE patients.
• The sCLEC-2xDD/PLT formula demonstrated utility in differentiating MVT from infection or non-thrombotic conditions via ROC analysis.

Conclusions:

• sCLEC-2 is a valuable biomarker for the diagnosis of thrombosis.
• Formulas incorporating sCLEC-2 with platelet count or D-dimer levels enhance the diagnostic accuracy for thrombotic diseases.
• ROC analysis confirms the utility of these combined markers in distinguishing thrombotic from non-thrombotic conditions.