Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stimulation of parathyroid hormone secretion by phorbol esters is associated with a decrease of cytosolic calcium.

R Muff, J A Fischer

    FEBS Letters
    |January 6, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pathogenic SCN2A variants cause early-stage dysfunction in patient-derived neurons.

    Human molecular genetics·2023
    Same author

    2D Raman, ATR-FTIR, WAXD, SAXS and DSC data of PET mono- and PET/PA6 bicomponent filaments.

    Data in brief·2021
    Same author

    Reduced-dimensionality-induced helimagnetism in iron nanoislands.

    Nature communications·2014
    Same author

    Endogenous α-calcitonin-gene-related peptide promotes exercise-induced, physiological heart hypertrophy in mice.

    Acta physiologica (Oxford, England)·2014
    Same author

    Enhanced vascular responses to adrenomedullin in mice overexpressing receptor-activity-modifying protein 2.

    Circulation research·2005
    Same author

    Receptors for calcitonin gene-related peptide, adrenomedullin, and amylin: the contributions of novel receptor-activity-modifying proteins.

    Receptors & channels·2003

    High extracellular calcium normally inhibits parathyroid hormone (PTH) secretion. Protein kinase C activation by TPA enhances PTH secretion and lowers cytosolic calcium levels in bovine parathyroid cells.

    Area of Science:

    • Endocrinology
    • Cell Signaling

    Background:

    • Calcium typically inhibits parathyroid hormone (PTH) secretion, correlating with increased cytosolic calcium ([Ca]i).
    • High extracellular calcium reduces diglyceride levels and protein kinase C (PKC) activity.

    Purpose of the Study:

    • To investigate the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA), a PKC activator, on [Ca]i and PTH secretion.
    • To explore the role of PKC in regulating PTH secretion and calcium homeostasis.

    Main Methods:

    • Utilized dispersed bovine parathyroid cells.
    • Administered TPA at varying extracellular calcium concentrations (1.5 mM and 0.5 mM).
    • Measured cytosolic calcium concentration ([Ca]i) and PTH secretion.

    Main Results:

    Related Experiment Videos

    • At 1.5 mM calcium, TPA enhanced PTH secretion and significantly reduced [Ca]i (from 639 nM to 335 nM).
    • TPA was ineffective at 0.5 mM calcium.
    • TPA suppressed the calcium-induced rise in [Ca]i.

    Conclusions:

    • TPA stimulates PTH secretion, likely through PKC activation.
    • The observed decrease in [Ca]i may be a downstream effect of PKC activation and altered diglyceride availability.