Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammatory Response01:28

Inflammatory Response

1.9K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
1.9K
T Cell Types and Functions01:24

T Cell Types and Functions

959
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
959
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

11.0K
The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
11.0K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K
Skin Cancer01:30

Skin Cancer

3.9K
Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-Applied Daylight Photodynamic Therapy: A Paradigm Shift?

International journal of molecular sciences·2025
Same author

Effect of Combination of Blue and Red Light with Terbinafine on Cell Viability and Reactive Oxygen Species in Human Keratinocytes: Potential Implications for Cutaneous Mycosis.

International journal of molecular sciences·2024
Same author

Determining the Best Noninvasive Test for Peripheral Arterial Disease Diagnosis to Predict Diabetic Foot Ulcer Healing in Patients Following Endovascular Revascularization.

Healthcare (Basel, Switzerland)·2024
Same author

Physical Treatments and Therapies for Androgenetic Alopecia.

Journal of clinical medicine·2024
Same author

Effects of RF Electric Currents on Hair Follicle Growth and Differentiation: A Possible Treatment for Alopecia.

International journal of molecular sciences·2024
Same author

Understanding Active Photoprotection: DNA-Repair Enzymes and Antioxidants.

Life (Basel, Switzerland)·2024

Related Experiment Video

Updated: Jun 10, 2025

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

16.3K

Radiofrequency Currents Modulate Inflammatory Processes in Keratinocytes.

Elena Toledano-Macías1, María Antonia Martínez-Pascual1, Almudena Cecilia-Matilla2

  • 1Photobiology and Bioelectromagnetic Lab, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Hospital Ramón y Cajal, Crta. Colmenar Viejo, km. 9.100, 28034 Madrid, Spain.

International Journal of Molecular Sciences
|October 16, 2024
PubMed
Summary

Capacitive Resistive Electric Transfer (CRET) therapy enhances keratinocyte proliferation and modulates inflammatory cytokine release. This physical therapy activates key signaling pathways, offering a novel approach to wound healing and inflammation control.

Keywords:
EGFRERK1/2MMPsNF-κBcytokineinflammationkeratinocytephysical therapiesradiofrequency

More Related Videos

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
08:45

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

5.2K
Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis
07:08

Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis

Published on: August 31, 2022

1.6K

Related Experiment Videos

Last Updated: Jun 10, 2025

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

16.3K
Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
08:45

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

5.2K
Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis
07:08

Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis

Published on: August 31, 2022

1.6K

Area of Science:

  • Biomedical Engineering
  • Dermatology
  • Wound Healing Research

Background:

  • Keratinocytes are crucial for wound inflammation and regeneration, producing cytokines that modulate the process.
  • Previous research suggests radiofrequency (RF) currents in Capacitive Resistive Electric Transfer (CRET) therapy can influence keratinocyte proliferation and cytokine production.
  • The biological mechanisms underlying the anti-inflammatory effects of physical therapies like CRET require further elucidation.

Purpose of the Study:

  • To investigate the impact of subthermal CRET on human keratinocyte (HaCat) proliferation.
  • To analyze CRET's effect on the production of key cytokines (IL-8, MCP-1, RANTES, IL-6, IL-11) and TNF-α.
  • To examine CRET-induced changes in the expression and activation of MMP9, MMP1, NF-κB, ERK1/2, and EGFR.

Main Methods:

  • Human keratinocytes (HaCat) were exposed to an intermittent 448 kHz CRET signal under subthermal conditions.
  • Cell proliferation was assessed using the XTT assay.
  • Cytokine and TNF-α levels were quantified via ELISA.
  • NF-κB activation was visualized using immunofluorescence.
  • Expression and activation of MMP9, MMP1, ERK1/2, and EGFR were determined by immunoblotting.

Main Results:

  • CRET significantly increased keratinocyte proliferation compared to controls.
  • A transient increase in MCP-1, TNF-α, and IL-6 release was observed, alongside a decrease in IL-8.
  • CRET treatment led to modifications in MMP expression and activation of EGFR, NF-κB, and ERK1/2 proteins.

Conclusions:

  • CRET therapy effectively modulates human keratinocyte proliferation and inflammatory cytokine profiles.
  • The observed effects are mediated through the EGF receptor and the ERK1/2/NF-κB signaling pathway.
  • CRET shows potential for modulating inflammatory responses in human keratinocytes, relevant for wound regeneration.