TNFα-Related Chondrocyte Inflammation Models: A Systematic Review

Area of Science:

• Biomedical Engineering
• Immunology
• Rheumatology

Background:

• Tumor necrosis factor alpha (TNFα) is a key cytokine in joint diseases.
• Numerous models exist for TNFα-induced cartilage inflammation, but lack standardization.
• Differences in model construction hinder result comparison across studies.

Purpose of the Study:

• To systematically review and compare existing TNFα chondrocyte inflammation models.
• To analyze model construction parameters, advantages, disadvantages, and analytical methods.
• To provide a basis for comparing current and future studies.

Main Methods:

• Systematic review and comparison of TNFα chondrocyte inflammation models.
• Analysis based on cell source, cytokine concentration, stimulation time, and mechanical stimulation.
• Categorization into monolayer-based, construct-based, explant-based, and miscellaneous models.

Main Results:

• Monolayer-based models are most common (42.86%).
• 10 ng/mL TNFα and passage 1 chondrocytes are frequently used.
• Human tissues are the primary source (51.43%), with limited use of mechanical stimulation.

Conclusions:

• Significant variations exist in TNFα chondrocyte inflammation model design.
• Standardization of experimental parameters is crucial for reliable research.
• Further experiments are needed to optimize and standardize these models.