Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Catenins01:23

Catenins

2.3K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.3K
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

3.2K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.2K
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

1.8K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.8K
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

6.6K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved...
6.6K
Structure of Cadherins01:25

Structure of Cadherins

3.3K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
3.3K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A genetic variant of adenylate cyclase 7 associated with ulcerative colitis shows impaired function and G-protein-coupled receptor signaling.

Human genetics·2026
Same author

Loss of B3GAT1/HNK-1 disrupts glioma-CD8<sup>+</sup> T cell immune synapse formation for immune escape.

International immunopharmacology·2025
Same author

Consequences of Adhesion Molecule Close Homolog of L1 Deficiency for Neurons and Glial Cells in the Mouse Spinal Cord After Injury.

Biomolecules·2025
Same author

The Upregulation of L1CAM by SVHRSP Mitigates Neuron Damage, Spontaneous Seizures, and Cognitive Dysfunction in a Kainic Acid-Induced Rat Model of Epilepsy.

Biomolecules·2025
Same author

NCAM2 promotes targeting of APP from the cell surface to BACE1-containing recycling endosomes.

Progress in neurobiology·2025
Same author

Depletion of Cell Adhesion Molecule L1 from Microglia and Macrophages Reduces Recovery After Spinal Cord Injury.

International journal of molecular sciences·2025
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Jun 10, 2025

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

9.1K

Functional Relationships between L1CAM, LC3, ATG12, and Aβ.

Gabriele Loers1, Ute Bork1, Melitta Schachner2

  • 1Zentrum für Molekulare Neurobiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.

International Journal of Molecular Sciences
|October 16, 2024
PubMed
Summary
This summary is machine-generated.

The adhesion molecule L1 aids in clearing toxic amyloid-beta (Aβ) protein aggregates in Alzheimer's disease models by facilitating autophagy, suggesting L1 enhancement as a potential therapeutic strategy.

Keywords:
Alzheimer’s diseaseAβL1CAMLC3LIRautophagy

More Related Videos

Correlative Light and Electron Microscopy to Study Microglial Interactions with &#946;-Amyloid Plaques
10:52

Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques

Published on: June 1, 2016

11.3K
Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
11:53

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Published on: March 31, 2023

1.1K

Related Experiment Videos

Last Updated: Jun 10, 2025

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

9.1K
Correlative Light and Electron Microscopy to Study Microglial Interactions with &#946;-Amyloid Plaques
10:52

Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques

Published on: June 1, 2016

11.3K
Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
11:53

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Published on: March 31, 2023

1.1K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Abnormal brain protein accumulations, such as amyloid-beta (Aβ), are hallmarks of aging and neurodegenerative diseases like Alzheimer's disease (AD).
  • Autophagy is a crucial cellular mechanism for degrading aggregated proteins, and its dysfunction contributes to neurodegeneration.
  • The adhesion molecule L1 has been shown to interact with microtubule-associated protein 1 light-chain 3 (LC3) and reduce Aβ plaque load in AD models.

Purpose of the Study:

  • To investigate the role of the adhesion molecule L1 in the autophagy-mediated clearance of aggregated amyloid-beta (Aβ).
  • To determine the specific interactions of L1 with autophagy-related proteins in the context of Aβ degradation.

Main Methods:

  • Investigated the interaction of L1 with autophagy-related protein 12 (ATG12) using its LC3 interacting region (LIR) domain.
  • Examined the interaction of L1 with p62/SQSTM1, a ubiquitin-binding protein.
  • Assessed the role of L1 in the transport of Aβ to autophagosomes for degradation.

Main Results:

  • L1 interacts with ATG12 via its LIR domain, facilitating the autophagic pathway.
  • Aβ bound to L1 is effectively transported to autophagosomes, leading to its clearance.
  • Evidence suggests the p62/SQSTM1 pathway also contributes to Aβ elimination, potentially mediated by ubiquitinated L1 fragments.

Conclusions:

  • L1 plays a significant role in the autophagy-dependent clearance of aggregated Aβ.
  • The interaction of L1 with ATG12 and its role in Aβ transport highlight its potential as a therapeutic target.
  • Enhancing L1 function may offer a novel therapeutic strategy for managing Alzheimer's disease and other proteinopathies.