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Compact Laser-Induced Fluorescence Detector with Adjustable Laser Focal Spot for Multiple Purposes.

Zihe Xu1,2, Xi Chen3, Fangwu Liu1

  • 1Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China.

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A new compact laser-induced fluorescence (LIF) detector was developed for sensitive molecular detection. This miniaturized system offers broad applicability across various analytical platforms, including space-based systems.

Keywords:
adjustableepifluorescencehandheldlaser-induced fluorescencelimit of detection

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Area of Science:

  • Analytical Chemistry
  • Biophotonics
  • Instrumentation Engineering

Background:

  • Increasing demand for miniaturized laser-induced fluorescence (LIF) detection systems across research fields.
  • Need for adaptable detection systems for diverse targets like capillaries, PCR tubes, and microfluidic chips.

Purpose of the Study:

  • To develop a compact, cost-effective, and highly sensitive LIF detector.
  • To optimize excitation source, wavelength, and power for enhanced detection limits.
  • To demonstrate the detector's integration and performance in various analytical systems, including space applications.

Main Methods:

  • Development of a compact LIF detector using a laser diode and photodiode with adjustable focal spot.
  • Implementation of background fluorescence correction, adaptive dynamic range adjustment, and constant laser power control.
  • Experimental investigation of excitation power's influence on detection limits and optimization of light source (LED/LD) and wavelength (487/450 nm).

Main Results:

  • Construction of a fully integrated, modular epifluorescence LIF detector (40 × 22 × 38 mm³) costing USD 320.
  • Achieved a detection limit of 0.4 nM for fluorescein sodium.
  • Successful integration into a nucleic acid detection system on the Chinese Space Station (CSS) and testing with PCR tubes and capillaries.

Conclusions:

  • The developed compact LIF detector meets the demand for miniaturized analytical systems.
  • The system demonstrates broad practicality, adaptability, and high sensitivity for diverse applications.
  • This technology holds potential for advanced molecular detection in various environments, including space exploration.