Training Protocol for Nellore Cattle in Respirometry Flow Trials Using Non-Ventilated Facial Mask

Area of Science:

• Animal Science
• Physiology
• Behavioral Science

Background:

• Flow respirometry requires animal cooperation with restraint and specialized equipment.
• Identifying suitable animals for such assays is crucial for data quality.

Purpose of the Study:

• To develop and evaluate a training protocol for Nellore cattle to improve cooperation in flow respirometry.
• To identify less reactive cattle suitable for mask-based physiological measurements.

Main Methods:

• A 127-day training protocol involving environmental acclimatization and positive reinforcement was applied to 30 Nellore males.
• Physiological and behavioral responses to restraint and facial mask use were monitored.
• Principal component analysis was used to differentiate animal reactivity levels.

Main Results:

• The training protocol effectively enhanced cattle cooperation with restraint and mask use.
• Less reactive animals demonstrated greater acceptance of handling and mask tolerance.
• The least reactive cattle tolerated mask use for extended periods without significant physiological changes.

Conclusions:

• A tailored training protocol can successfully select cooperative Nellore cattle for flow respirometry.
• This approach ensures reliable physiological data collection without adverse behavioral or physiological effects.