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Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

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Doramectin Induces Apoptosis in B16 Melanoma Cells.

Megan S Crotts^1,2, Jena C Jacobs^1,2, Robert W Baer^1,2

¹Department of Biochemistry, Kirksville College of Osteopathic Medicine, A.T. Still University of Health Sciences, Kirksville, Missouri, USA.

Anti-Cancer Agents in Medicinal Chemistry

|October 16, 2024

Summary

Doramectin treatment reduced melanoma cell growth and induced apoptosis, a cell death pathway distinct from autophagy. Further research is warranted for its potential as an anti-melanoma agent.

Keywords:

Melanoma apoptosis autophagy cancer cell death.doramectin ivermectin

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Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

Area of Science:

Oncology
Cell Biology
Pharmacology

Background:

Metastatic melanoma often resists conventional apoptosis-inducing therapies.
Alternative cell-death pathways are being explored for melanoma treatment.
Doramectin has shown efficacy in neuroblastoma by inhibiting growth via autophagy.

Purpose of the Study:

To investigate if doramectin induces autophagy in B16F10 melanoma cells.
To explore doramectin's mechanism of action in melanoma.
To evaluate doramectin as a potential anti-cancer agent for melanoma.

Main Methods:

B16F10 melanoma cells were treated with doramectin.
MTT analysis assessed cell viability and growth inhibition.
MDC analysis, flow cytometry, and TUNEL assays were used to detect autophagy and cell death.
Cells were also treated with a combination of doramectin and a cell-death inhibitor.

Main Results:

Doramectin treatment significantly decreased melanoma cell growth.
Apoptotic morphology was observed in doramectin-treated cells.
Flow cytometry indicated apoptosis and some necrosis as primary cell death mechanisms, not autophagy.
MDC analysis did not detect increased autophagy.

Conclusions:

Doramectin induces apoptosis and necrosis in melanoma cells, not autophagy.
This represents a novel cell-death mechanism for melanoma treatment.
Doramectin warrants further investigation as a potential anti-melanoma therapeutic agent.