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Related Experiment Video

Updated: Jun 10, 2025

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
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Amur Grape VaMYB4a-VaERF054-Like Module Regulates Cold Tolerance Through a Regulatory Feedback Loop.

Kai Lv1, Yaping Xie1, Qinhan Yu2

  • 1College of Enology and Horticulture, Ningxia University, Yinchuan, Ningxia, China.

Plant, Cell & Environment
|October 16, 2024
PubMed
Summary

Grapevine cold tolerance is enhanced by VaERF054-like, a gene regulated by VaMYB4a. This interaction improves cold resistance through ethylene and CBF pathways, aiding development of hardier grape varieties.

Keywords:
VaERF054‐likeVaMYB4aVitis amurensiscold stress responsesethylene

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Area of Science:

  • Plant Science
  • Molecular Biology
  • Agricultural Science

Background:

  • Cold stress significantly impacts grapevine growth, development, and productivity.
  • Understanding the molecular mechanisms of grapevine cold stress response is crucial for agricultural advancement.

Purpose of the Study:

  • To characterize the role of an APETALA2/ethylene response factor (AP2/ERF) gene, VaERF054-like, in grapevine cold tolerance.
  • To investigate the molecular interaction between VaMYB4a and VaERF054-like in regulating cold stress response.

Main Methods:

  • Gene expression analysis of VaERF054-like under cold stress.
  • Promoter binding assays to determine VaMYB4a's interaction with VaERF054-like.
  • Ethylene precursor and inhibitor treatments to assess pathway involvement.
  • Overexpression studies in Vitis vinifera 'Chardonnay' calli and transgenic lines.
  • In vivo and in vitro interaction studies between VaMYB4a and VaERF054-like.

Main Results:

  • VaMYB4a directly binds and activates the VaERF054-like promoter, increasing its expression.
  • VaERF054-like enhances cold tolerance by modulating ethylene and CBF signaling pathways.
  • Overexpression of VaERF054-like confers increased freezing stress tolerance in grape calli and transgenic plants.
  • VaMYB4a and VaERF054-like exhibit synergistic interaction, boosting cold tolerance via feedback regulation.

Conclusions:

  • VaERF054-like is a key regulator of cold stress tolerance in grapes, acting downstream of VaMYB4a.
  • The VaMYB4a-VaERF054-like regulatory module offers a promising target for developing cold-resistant grape varieties.
  • Findings provide novel insights into grapevine's molecular response to cold stress.