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FERONIA controls ABA-mediated seed germination via the regulation of CARK1 kinase activity
Xiaoxiao Wang1, Jianwei Liu2, Mingtao Wang1
1Key Laboratory of Plant Design, National Key Laboratory of Plant Molecular Genetics, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China; University of Chinese Academy of Sciences, Beijing 100190, China.
Abstract:
Seed germination is the initial step of the whole life cycle for an individual plant, and thus it needs to be tightly controlled to avoid plant growth under unfavorable conditions. Here, we report that FERONIA (FER), a receptor-like kinase, controls early seed germination under ABA conditions. FER interacts with and phosphorylates cytosolic ABA receptor kinase 1 (CARK1) protein, a receptor-like cytoplasmic kinase (RLCK) that modulates ABA signaling. In both the fer-4 and cark1 mutants, ABA-triggered SNF1-related protein kinase 2 (SnRK2) activation and ABI5 protein accumulation are attenuated. FER phosphorylates the Ser233 and Thr234 residues of CARK1, and the CARK1 protein with the substitutions of these two residues with Ala exhibits a reduced kinase activity and fails to rescue the increased seed germination rate of the cark1 mutant under ABA conditions. Collectively, our study not only uncovers an RLCK protein that functions downstream of FER but also provides a mechanistic insight into ABA-mediated early seed germination regulation by the FER-CARK1 module.
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