Related Experiment Video
Updated: Jun 10, 2025
11:05
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
Published on: January 21, 2015
33.2K
NAIRDB: a database of Fourier transform infrared (FTIR) data for nucleic acids
Elsa Balduzzi1, Frédéric Geinguenaud2, Dominik Sordyl3
1Laboratoire Léon Brillouin, UMR 12 CEA/CNRS, Bâtiment 563, Site de Saclay, 91191 Gif-sur-Yvette, France.
Nucleic Acids Research
|October 16, 2024
Summary
The Nucleic Acid InfraRed Data Bank (NAIRDB) provides free access to Fourier transform infrared (FTIR) spectral data for nucleic acids. This curated database supports research by offering diverse spectra and experimental details for DNA and RNA molecules.
Area of Science:
- Biophysics
- Molecular Biology
- Bioinformatics
Background:
- Fourier transform infrared (FTIR) spectroscopy is a powerful technique for analyzing molecular structures.
- Nucleic acids (DNA, RNA) exhibit unique spectral fingerprints based on their composition and structure.
- A centralized, curated database for nucleic acid FTIR data is lacking.
Purpose of the Study:
- To establish a comprehensive public repository for archival and distribution of nucleic acid FTIR spectral data.
- To facilitate research in nucleic acid structure and function through accessible spectral information.
- To encourage data sharing and collaboration within the scientific community.
Main Methods:
- Compilation of FTIR spectral data for various nucleic acid molecules (DNA, RNA, hybrids, derivatives).
- Inclusion of experimental conditions, literature links, sequence data, and structural information (experimental and modeled).
- Expert validation and curation of all database entries for quality assurance.
Main Results:
- NAIRDB offers a searchable collection of validated FTIR spectra for diverse nucleic acids.
- The database allows searching by nucleic acid sequence similarity or spectral comparison.
- It provides detailed metadata including experimental parameters and structural context.
Conclusions:
- NAIRDB serves as a valuable resource for researchers studying nucleic acid structure and interactions.
- The database promotes data accessibility and standardization in nucleic acid spectroscopy.
- It is open for community contributions of new FTIR data for nucleic acids.
More Related Videos
Related Concept Videos
01:03IR Frequency Region: Fingerprint Region
788
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
788
01:19IR Spectrum
943
When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...
943
01:24IR Frequency Region: X–H Stretching
915
In IR spectroscopy, signals produced by the X−H bonds (such as C−H, O−H, or N−H) can be observed in the frequency range of 2700–4000 cm–1. The C−H stretching vibration forms sharp bands in the region 2850–3000 cm–1. The presence of the O−H stretching vibration leads to the forming of an absorption band in the frequency range 3650–3200 cm−1. At the same time, N−H stretching can be confirmed by absorption bands in...
915
01:09Infrared (IR) Spectroscopy: Overview
1.5K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
Different compounds display unique properties due to their...
1.5K
01:22IR Frequency Region: Alkyne and Nitrile Stretching
796
Both alkyne (C≡C) and nitrile (C≡N) functional groups contain triple bonds and show stretching absorptions around the wavenumber range of 2100 to 2300 cm−1 in the diagnostic region of the IR spectra.
Comparing the stretching vibrational frequency of C≡C triple bonds with that of double and single bonds, it is evident that C≡C triple bonds exhibit a higher stretching frequency than C=C double and C–C single bonds. Similarly, the C≡N triple bond...
Comparing the stretching vibrational frequency of C≡C triple bonds with that of double and single bonds, it is evident that C≡C triple bonds exhibit a higher stretching frequency than C=C double and C–C single bonds. Similarly, the C≡N triple bond...
796
01:13Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
295
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
The ATR process begins by directing a beam...
295