Response processes to water quality changes driven by the dynamic regeneration of the surface microlayer film in...

The freshwater surface microlayer film (SMF) is a dynamically evolving micro-ecosystem closely related to water quality and microbial growth in water. However, the mechanisms of dynamic regeneration of SMF after their destruction and their impacts on the aquatic environment are still largely unknown. Herein, we modeled the dynamic processes of SMF destruction and recovery in the natural environment by constructing a water-SMF ecosystem. Chemical and biological changes in the dynamic regeneration of SMF were investigated. The results showed that the dynamic regeneration of SMF was very fast, with a regeneration thickness of up to 300 ± 50 μm in two days, and at the same time, it would rapidly enrich organic matter and Fe ions. In addition, the cyclic dynamic regeneration process of SMF is significantly correlated with the surge metabolic growth of microorganisms associated with organic matter metabolism (e.g., Methylophilus, Nevskia) and iron-redox-associated (e.g., Curvibacter). The experimental results suggest that the microbial-mediated process of iron-organic matter coupled oxidation-reduction in SMF may be another important mechanism driving water quality changes. Overall, our study provides valuable theoretical guidance for predicting changes in water quality in slow-flowing water bodies.