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Updated: Jun 10, 2025
Skeletal Muscle Gender Dimorphism from Proteomics
Published on: December 14, 2011
Gender- and Age-Based Characterization and Comparison of the Murine Primary Peritoneal Mesothelial Cell Proteome
Zhikun Wang1,2, Yueying Liu1,2, Reihaneh Safavisohi1,2
1Department of Chemistry & Biochemistry, University of Notre Dame, Notre Dame IN 46556.
Abstract:
Organs in the abdominal cavity are covered by a peritoneal membrane, which is comprised of a monolayer of mesothelial cells (MC). Diseases involving the peritoneal membrane include peritonitis, primary cancer (mesothelioma), and metastatic cancers (ovarian, pancreatic, colorectal). These diseases have gender- and/or age-related pathologies; however, the impact of gender and age on the peritoneal MC is not well evaluated. To address this, we identified and characterized gender- and age-related differences in the proteomes of murine primary peritoneal MC. Primary peritoneal MC were isolated from young female (FY) or male (MY) mice (3-6 months) and aged female (FA) or male (MA) mice (20-23 months), lysed, trypsin digested using S-Traps, then subjected to bottom-up proteomics using an LC-Orbitrap mass spectrometer. In each cohort, we identified >1000 protein groups. Proteins were categorized using Gene Ontology and pairwise comparisons between gender and age cohorts were conducted. This study establishes baseline information for studies on peritoneal MC in health and disease at two physiologic age/gender points. Segregation of the data by gender and age could reveal novel factors to specific disease states involving the peritoneum. [This in vitro primary cell model has utility for future studies on the interaction between the mesothelium and foreign materials.].
Insights
This study reveals key proteomic differences in peritoneal mesothelial cells (MC) based on gender and age in mice. Understanding these variations is crucial for studying peritoneal diseases like cancer.
Area of Science:
- Proteomics
- Cell Biology
- Oncology
Background:
- The peritoneal membrane, lined by mesothelial cells (MC), is involved in various diseases, including cancers.
- Existing research on peritoneal diseases often overlooks the influence of gender and age on MC.
- This knowledge gap hinders a comprehensive understanding of peritoneal pathologies.
Purpose of the Study:
- To investigate and characterize gender- and age-related proteomic differences in primary murine peritoneal MC.
- To establish a baseline understanding of peritoneal MC biology across different demographic groups.
- To identify potential factors influencing peritoneal health and disease states.
Main Methods:
- Primary peritoneal MC were isolated from young and aged female and male mice.
- Proteomic analysis was performed using liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap).
- Proteins were identified, quantified, and categorized using Gene Ontology for comparative analysis.
Main Results:
- Over 1000 protein groups were identified in each experimental cohort.
- Significant proteomic differences were observed between male and female, and young and aged MC.
- Data segregation by gender and age provided novel insights into MC characteristics.
Conclusions:
- This research provides foundational proteomic data for peritoneal MC across distinct gender and age groups.
- The findings offer a basis for future investigations into gender- and age-specific peritoneal diseases.
- The characterized cell model is valuable for studying mesothelium interactions in various conditions.
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