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Curcumin and Vitamin D Reduce HMGB-1 mRNA Levels in Mice Infected with Salmonella typhi
Ami Febriza1,2, Hasta Handayani Idrus2
1Department of Physiology, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Indonesia.
Background:
This study examined the effects of curcumin and vitamin D on high-mobility group box-1 (HMGB-1) mRNA expression in mice infected with Salmonella typhi.
Methods:
The experimental design allocated 40 mice, intraperitoneally infected with S. typhi, to pre- and post-test controls randomly divided into four groups (10 mice per group). Mice in group A were treated with the antibiotic levofloxacin (1.95 mg/kg once daily) as the positive control; group B mice were administered curcumin at a dose of 200 mg/kg body weight; group C mice were treated with a curcumin dose of 200 mg/kg BW and vitamin D; and group D mice received distilled water (placebo) as the negative control. The intervention was performed for 5 days. On day 10, HMGB-1 mRNA expression was measured, and the results were compared to those before the intervention.
Results:
HMGB-1 mRNA level in group C decreased significantly by 5.76-fold (95% confidence interval: 2.55, 8.98). In contrast, HMGB-1 mRNA levels did not decrease significantly in group B.
Conclusion:
These results suggest that the combination of curcumin and vitamin D reduced HMGB-1 mRNA levels in infected mice, highlighting the potential of this combination as an antimicrobial and anti-inflammatory agent.
Insights
The combination of curcumin and vitamin D significantly reduced high-mobility group box-1 (HMGB-1) mRNA levels in mice infected with Salmonella typhi. This suggests potential antimicrobial and anti-inflammatory benefits.
Area of Science:
- Biochemistry
- Immunology
- Pharmacology
Background:
- Salmonella typhi infection poses a significant health challenge.
- High-mobility group box-1 (HMGB-1) is implicated in inflammatory responses during infection.
- Curcumin and vitamin D are investigated for their potential therapeutic properties.
Purpose of the Study:
- To investigate the effects of curcumin and vitamin D on HMGB-1 mRNA expression in Salmonella typhi-infected mice.
- To evaluate the combined efficacy of curcumin and vitamin D as an antimicrobial and anti-inflammatory intervention.
Main Methods:
- Forty mice infected with Salmonella typhi were divided into four groups.
- Groups received levofloxacin (positive control), curcumin, curcumin plus vitamin D, or placebo for 5 days.
- HMGB-1 mRNA expression was measured on day 10 post-infection.
Main Results:
- The group treated with both curcumin and vitamin D showed a significant 5.76-fold decrease in HMGB-1 mRNA levels.
- Curcumin treatment alone did not result in a significant reduction of HMGB-1 mRNA levels.
Conclusions:
- The combination of curcumin and vitamin D effectively reduces HMGB-1 mRNA expression in Salmonella typhi-infected mice.
- This combination demonstrates potential as a novel antimicrobial and anti-inflammatory therapeutic strategy.
